Abstract
Cell-free systems from the bacteria Escherichia coli and Klebsiella planticola that were incubated with 13C labeled pyruvate and D- glyceraldehyde synthesized 5-hydroxypentane-2,3-dione (laurencione) along with 1-deoxy-D-threo-pentulose (1-deoxy-D-xylulose). Both compounds showed identical labeling patterns, indicating that the C5 skeletons were derived from the condensation of (hydroxyethyl)thiamin on D-glyceraldehyde. Conversion of [5,5-2H2]deoxyxylulose into laurencione by a cell-free system from E. coli showed that the α-dione is obtained from the pentulose by water elimination.
Original language | English |
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Pages (from-to) | 6185-6188 |
Number of pages | 4 |
Journal | Tetrahedron Letters |
Volume | 39 |
Issue number | 34 |
DOIs | |
Publication status | Published - 20 Aug 1998 |
Externally published | Yes |