TY - JOUR
T1 - A new copper (II)-imidazole derivative effectively inhibits replication of denv-2 in vero cell
AU - Sucipto, Teguh Hari
AU - Churrotin, Siti
AU - Setyawati, Harsasi
AU - Martak, Fahimah
AU - Mulyatno, Kris Cahyo
AU - Amarullah, Ilham Harlan
AU - Kotaki, Tomohiro
AU - Kameoka, Masanori
AU - Yotopranoto, Subagyo
AU - Soegijanto, Soegeng
N1 - Publisher Copyright:
© 2018, Obafemi Awolowo University. All rights reserved.
PY - 2018
Y1 - 2018
N2 - Background: Dengue is a kind of infectious disease that was distributed in the tropical and sub-tropical areas. To date, there is no clinically approved dengue vaccine or antiviral for humans, even though there have been great efforts towards this end. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the complex compounds, copper(II)-imidazole derivatives are of interest because of their biological and medicinal benefits. Materials and Methods: In the present study, antiviral activity of [Cu(2,4,5-triphenylimidazole)2]n, was evaluated against different stages of dengue virus type 2 (DENV-2) replication in Vero cell using focus forming unit reduction assay and quantitative ELISA. Results: [Cu(2,4,5-triphenylimidazole)2]n inhibited DENV-2 replication in Vero cells with IC50 = 2.3 μg/ml and SI= 19.42 when cells were treated 2 days after virus infection, whereas its CC50 for cytotoxicity to Vero cells was 44.174 μg/ml. Conclusion: The compound has high anti-DENV2 activity, less toxicity, and a high possibility to be considered a drug candidate.
AB - Background: Dengue is a kind of infectious disease that was distributed in the tropical and sub-tropical areas. To date, there is no clinically approved dengue vaccine or antiviral for humans, even though there have been great efforts towards this end. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the complex compounds, copper(II)-imidazole derivatives are of interest because of their biological and medicinal benefits. Materials and Methods: In the present study, antiviral activity of [Cu(2,4,5-triphenylimidazole)2]n, was evaluated against different stages of dengue virus type 2 (DENV-2) replication in Vero cell using focus forming unit reduction assay and quantitative ELISA. Results: [Cu(2,4,5-triphenylimidazole)2]n inhibited DENV-2 replication in Vero cells with IC50 = 2.3 μg/ml and SI= 19.42 when cells were treated 2 days after virus infection, whereas its CC50 for cytotoxicity to Vero cells was 44.174 μg/ml. Conclusion: The compound has high anti-DENV2 activity, less toxicity, and a high possibility to be considered a drug candidate.
KW - Antiviral activity
KW - DENV-2
KW - Vero cell
KW - [Cu(2,4,5-triphenylimidazole)]
UR - http://www.scopus.com/inward/record.url?scp=85043333034&partnerID=8YFLogxK
U2 - 10.2101/Ajid.12v1S.17
DO - 10.2101/Ajid.12v1S.17
M3 - Article
AN - SCOPUS:85043333034
SN - 2006-0165
VL - 12
SP - 116
EP - 119
JO - African Journal of Infectious Diseases
JF - African Journal of Infectious Diseases
IS - Special Issue 1
ER -
