Absence of anti-Staphylococcus aureus (MRSA) activity of secondary metabolite actinomycetes associated sponges from Pulau Panjang, Indonesia

Objective: To study the anti-Staphylococcus aureus MRSA of the secondary metabolite actinomycetes associated sponges from Pulau Panjang, Central Java, Indonesia. Materials and Methods: The sponge samples were taken by scuba diving in Pulau Panjang, and the sponge was identified using external morphology as well as identification of spicules from the fresh preparation. Isolation of actinomycetes associated with sponge was conducted by means of direct plating. The symbion bacteria were planted (scratched in a circular way) on agar medium Zobell 2216E, medium actinomycetes using the existing method, namely, S.aureus MRSA and E. coli MRSA. Results: The results of identification from 6 sponges were Phakettia euctimenna, Gelliodes fibolatus, Lamellodysidea herbacea, Clathria reindwardtii and Callyspongia sp, while antibacterial assay for the secondary metabolite actinomycetes associated sponges showed negative results. Conclusion: Secondary metabolite actinomycetes associated sponges from Pulau Panjang were not found to have antibacterial activity against S. aureus MRSA.