E-bike (Pygmy) as a two-wheeled vehicle used for urban public transportation users in traveling the distance from home to bus stop/station, bus stop/station to the office, or vice versa, needs to be designed lightly and portable with foldable capabilities. In fulfilling the repeated use of e-bikes, it is necessary to analyze the frame in a dynamic state or frame condition when used to determine when fatigue occurs or to determine the age of the frame. In the study of two-wheeled vehicle frames in a dynamic state, the frame is analyzed in the worst condition when it is used, there are the maximum braking of the front wheel and maximum acceleration occurs. In the existing condition of AISI 1020 Steel material with an e-bike mass of 21kg, it is found that mechanical fatigue will occur after experiencing 1000 cycles at 80kg rider load as the maximum load can withstand. The use of other alternative materials, aluminum 7075-T6 obtained mechanical fatigue of 1.5 x 107 cycles at the same rider load, 80kg with a smaller e-bike mass of 16.270kg but followed by an increase in material procurement costs by 2 times more than AISI 1020 steel.