Piper retrofractum Vahl., which belongs to the family Piperaceae, is geographically dispersed in tropical region including Indonesia. They are well-known spice possessing high medicinal properties. This study aimed to determine the antioxidant activity of P. retrofractum fruit, extracted with different solvents (methanol, ethyl acetate, n-hexane) using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. This research was carried out using different concentrations of methanol, ethyl acetate, and n-hexane extracts, (0, 5, 15, 30, 45, 60 ppm). Ascorbic acid was also used as positive antioxidant control. The percentage of inhibition and IC50 were measured. The results showed that the DPPH free radicals were scavenged by all plant extracts in a concentration dependent manner. Moreover, the IC50 values for DPPH radicals with methanol, ethyl acetate and n-hexane extract of the P. retrofractum Vahl. were found to be 101.74; 66.12 and 57.66 ppm, respectively. Interestingly, the IC50 value of n-hexane extract (57.66 ppm) was lower than ascorbic acid (66.12 ppm), indicating that n-hexane extract was a more potent scavenger of free radicals than methanol and ethyl acetate extracts. Taken together, our results suggested that n-hexane extract of P. Retrofractum Vahl. might contain potential antioxidant compounds.

Original languageEnglish
Title of host publicationProceeding of International Biology Conference 2016
Subtitle of host publicationBiodiversity and Biotechnology for Human Welfare
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735415287
Publication statusPublished - 26 Jun 2017
Event3rd International Biology Conference 2016: Biodiversity and Biotechnology for Human Welfare, IBOC 2016 - Surabaya, Indonesia
Duration: 15 Oct 2016 → …

Publication series

NameAIP Conference Proceedings
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616


Conference3rd International Biology Conference 2016: Biodiversity and Biotechnology for Human Welfare, IBOC 2016
Period15/10/16 → …


  • DPPH assay
  • Piper retrofractum Vahl
  • antioxidant


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