TY - JOUR
T1 - Antioxidant and antibacterial activities of Ischaemum indicum leaves extracted using different solvents
AU - Auwaliyah, Fithrotul
AU - Rizqi, Hamdan Dwi
AU - Purnomo, Adi Setyo
AU - Fatmawati, Sri
N1 - Publisher Copyright:
© 2019 Penerbit UTM Press.
PY - 2019/5
Y1 - 2019/5
N2 - The aim of this study was to evaluate antioxidant and antibacterial activities of Ischaemum indicum leaves extracts based on different polarity solvents: water, methanol, ethyl acetate, dichloromethane, and n-hexane. The antioxidant activity was evaluated using ABTS and DPPH methods, while the antibacterial activity was determined by broth dilution method against Pseudomonas aeruginosa and Bacillus subtilis. The results showed that water and methanol extracts of I. indicum gave the highest antioxidant activity in ABTS assay with an IC50 73.80 and 46.32 μg/mL, respectively. On the other hand, The IC50 on DPPH assay for water extracts was 117.51 μg/mL, this value was higher than that of methanol extract with no inhibition. Methanol and water extracts showed the antibacterial activity against B. subtilis with inhibition of 48.62 and 45.11%, whereas inhibition of P. aeruginosa were 31.95 and 30.31%, respectively. This study denoted that I. indicum is a new potential candidate as antioxidant source.
AB - The aim of this study was to evaluate antioxidant and antibacterial activities of Ischaemum indicum leaves extracts based on different polarity solvents: water, methanol, ethyl acetate, dichloromethane, and n-hexane. The antioxidant activity was evaluated using ABTS and DPPH methods, while the antibacterial activity was determined by broth dilution method against Pseudomonas aeruginosa and Bacillus subtilis. The results showed that water and methanol extracts of I. indicum gave the highest antioxidant activity in ABTS assay with an IC50 73.80 and 46.32 μg/mL, respectively. On the other hand, The IC50 on DPPH assay for water extracts was 117.51 μg/mL, this value was higher than that of methanol extract with no inhibition. Methanol and water extracts showed the antibacterial activity against B. subtilis with inhibition of 48.62 and 45.11%, whereas inhibition of P. aeruginosa were 31.95 and 30.31%, respectively. This study denoted that I. indicum is a new potential candidate as antioxidant source.
KW - ABTS
KW - Antibacterial
KW - Antioxidant
KW - DPPH
KW - Ischaemum indicum
UR - http://www.scopus.com/inward/record.url?scp=85078232113&partnerID=8YFLogxK
U2 - 10.11113/mjfas.v15n3.1037
DO - 10.11113/mjfas.v15n3.1037
M3 - Article
AN - SCOPUS:85078232113
SN - 2289-5981
VL - 15
SP - 436
EP - 440
JO - Malaysian Journal of Fundamental and Applied Sciences
JF - Malaysian Journal of Fundamental and Applied Sciences
IS - 3
ER -