Oligospermia is a male fertility issue defined as a low sperm concentration in the ejaculate. Normally the sperm concentration is 20-120 million/ml, while Oligospermia patients has sperm concentration less than 20 million/ml. Sperm test done in the fertility laboratory to determine oligospermia by checking fresh sperm according to WHO standards in 2010 [9]. The sperm seen in a microscope using a Neubauer improved counting chamber and manually count the number of sperm. In order to be counted automatically, this research made an automation system to analyse and count the sperm concentration called Automated Analysis of Sperm Concentration Counters (A2SC2) using Otsu threshold segmentation process and morphology. Data sperm used is the fresh sperm directly in the analysis in the laboratory from 10 people. The test results using A2SC2 method obtained an accuracy of 91%. Thus in this study, A2SC2 can be used to calculate the amount and concentration of sperm automatically.

Original languageEnglish
Article number012038
JournalIOP Conference Series: Materials Science and Engineering
Issue number1
Publication statusPublished - 28 Jan 2016
EventInternational Conference on Engineering and Technology for Sustainable Development, ICET4SD 2015 - Yogyakarta, Indonesia
Duration: 11 Nov 201512 Nov 2015


  • Concentration
  • Labelling
  • Oligospermia
  • Sperm
  • Threshold


Dive into the research topics of 'Automated Analysis of Human Sperm Number and Concentration (Oligospermia) Using Otsu Threshold Method and Labelling'. Together they form a unique fingerprint.

Cite this