Bacteria Isolation of Kerosene Contaminated Asphalt Waste (Asbuton)

Kerosene is a type of hydrocarbon commonly known as paraffin oil which is usually used as a home heating fuel, lamp and asphalt solvent. The presence of kerosene in the asphalt production can potentially cause environmental pollution, one of which affects soil conditions. Some bacteria that have specific ability to degrade hydrocarbon content in soil are called hydrocarbonoclastic bacteria. This study aims to determine the proportion of the presence of bacteria that have the potential to degrade hydrocarbons, in this case kerosene, in polluted soil at the location of one of the largest asphalt manufacturing companies in Indonesia and to determine the morphological characters of bacterial isolates. Based on the results of the study, Hydrocarbonoclastic bacteria isolated from asbuton had 5 dominant isolates and were identified by PCR analysis. The results of this study were that the five bacterial isolates were Bacillus sp. with 3 of them being Bacillus subtilis and 2 others being Bacillus cereus. This result has an Entiren stiffness of 99.62% - 100%. The type of bacteria Bacillus sp. are capable of reducing the Total Petroleum Hydrocarbon (TPH) and still able to survive and potentially degrade the TPH content in it.