Bamboo fibre processing: Insights into hemicellulase and cellulase substrate accessibility

Jiajia Fu, Gibson S. Nyanhongo, Carla Silva, Massimiliano Cardinale, Endry Nugroho Prasetyo, Chongwen Yu*, Artur Cavaco-Paulo, Georg M. Gübitz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


A biological approach for degumming bamboo substrates has been assessed. The ability of various commercially available enzymes, including cellulase, xylanase, pectinase and mannanase, to hydrolyze bamboo powders was investigated. In addition, a commercial cellulase preparation was applied onto bamboo fibre bundles obtained by natural retting. It was found that almost all enzymes applied can use bamboo material as a substrate. Mild autoclave pre-treatment can enhance reducing sugar yield from different enzyme treatments. A most pronounced effect was observed with cellulase treatment in which the hydrolysis degree increased 1.7 fold as measured by reducing sugars for autoclave pre-treated bamboo powders versus non-treated powders after only a short period of incubation. The combined treatment of hemicellulase preparations showed no effect on the hydrolysis of bamboo substrates. The effect of autoclave pre-treatment on cellulase-treated samples was confirmed by the increase of sugar yield, protein absorption as well as by the enhancement of surface modification and enzyme penetration observed by CLSM (confocal laser scanning microscopy). This work establishes a base for future studies to develop enzymatic hydrolysis of bamboo materials, making them suitable for textile processing.

Original languageEnglish
Pages (from-to)27-37
Number of pages11
JournalBiocatalysis and Biotransformation
Issue number1
Publication statusPublished - Feb 2012
Externally publishedYes


  • Autoclave
  • Bamboo
  • CLSM
  • Cellulase
  • Mannanase
  • Pectinase
  • Xylanase


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