TY - JOUR
T1 - Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility
AU - Nyanhongo, Gibson S.
AU - Rodríguez, Rosario Díaz
AU - Prasetyo, Endry Nugroho
AU - Caparrós, Cristina
AU - Ribeiro, Clarisse
AU - Sencadas, Vitor
AU - Lanceros-Mendez, Senentxu
AU - Acero, Enrique Herrero
AU - Guebitz, Georg M.
N1 - Funding Information:
This work has been supported by the Federal Ministry of Economy, Family and Youth (BMWFJ), the Federal Ministry of Traffic, Innovation and Technology (bmvit), the Styrian Business Promotion Agency SFG, the Standortagentur Tirol and ZIT – Technology Agency of the City of Vienna through the COMET-Funding Program managed by the Austrian Research Promotion Agency FFG and EU-NOVO Project.
PY - 2013
Y1 - 2013
N2 - Biocompatibility is a major challenge for successful application of many biomaterials. In this study the ability to coat chemically and enzymatically activated poly(L-lactic acid) (PLA) membranes with heat denatured human serum albumin to improve biocompatibility was investigated. PLA membranes hydrolyzed with NaOH or cutinase and then treated with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, hydrochloride (EDAC) as a heterobifunctional cross-linker promoted the coupling ACOOH groups on PLA membranes and ANH2 groups of heat denatured human serum albumin. This resulted in increased hydrophilicity (lowest water contact angles of 43° and 35°) and highest antioxidant activity (quenching of 79 μM and 115 μM tetramethylazobisquinone (TMAMQ) for NaOH and cutinase pretreated membranes, respectively). FTIR analysis of modified PLA membranes showed new peaks attributed to human serum albumin (amide bond, NH2 and side chain stretching) appearing within 3600-3000 cm 1 and 1700-1500 cm 1 (Fig. 3). MTT studies also showed that osteoblasts-like and MC-3T3-E1 cells viability increased 2.4 times as compared to untreated PLA membranes. The study therefore shows that this strategy of modifying the surfaces of PLA polymers could significantly improve biocompatibility.
AB - Biocompatibility is a major challenge for successful application of many biomaterials. In this study the ability to coat chemically and enzymatically activated poly(L-lactic acid) (PLA) membranes with heat denatured human serum albumin to improve biocompatibility was investigated. PLA membranes hydrolyzed with NaOH or cutinase and then treated with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, hydrochloride (EDAC) as a heterobifunctional cross-linker promoted the coupling ACOOH groups on PLA membranes and ANH2 groups of heat denatured human serum albumin. This resulted in increased hydrophilicity (lowest water contact angles of 43° and 35°) and highest antioxidant activity (quenching of 79 μM and 115 μM tetramethylazobisquinone (TMAMQ) for NaOH and cutinase pretreated membranes, respectively). FTIR analysis of modified PLA membranes showed new peaks attributed to human serum albumin (amide bond, NH2 and side chain stretching) appearing within 3600-3000 cm 1 and 1700-1500 cm 1 (Fig. 3). MTT studies also showed that osteoblasts-like and MC-3T3-E1 cells viability increased 2.4 times as compared to untreated PLA membranes. The study therefore shows that this strategy of modifying the surfaces of PLA polymers could significantly improve biocompatibility.
KW - Bioactive
KW - Cell viability
KW - Cytocompatibility
KW - Functionalization
KW - Human serum
KW - Polylactic acid membranes
UR - http://www.scopus.com/inward/record.url?scp=84885382734&partnerID=8YFLogxK
U2 - 10.1016/j.reactfunctpolym.2012.12.007
DO - 10.1016/j.reactfunctpolym.2012.12.007
M3 - Article
AN - SCOPUS:84885382734
SN - 1381-5148
VL - 73
SP - 1399
EP - 1404
JO - Reactive and Functional Polymers
JF - Reactive and Functional Polymers
IS - 10
ER -