Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility

Gibson S. Nyanhongo*, Rosario Díaz Rodríguez, Endry Nugroho Prasetyo, Cristina Caparrós, Clarisse Ribeiro, Vitor Sencadas, Senentxu Lanceros-Mendez, Enrique Herrero Acero, Georg M. Guebitz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Biocompatibility is a major challenge for successful application of many biomaterials. In this study the ability to coat chemically and enzymatically activated poly(L-lactic acid) (PLA) membranes with heat denatured human serum albumin to improve biocompatibility was investigated. PLA membranes hydrolyzed with NaOH or cutinase and then treated with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, hydrochloride (EDAC) as a heterobifunctional cross-linker promoted the coupling ACOOH groups on PLA membranes and ANH2 groups of heat denatured human serum albumin. This resulted in increased hydrophilicity (lowest water contact angles of 43° and 35°) and highest antioxidant activity (quenching of 79 μM and 115 μM tetramethylazobisquinone (TMAMQ) for NaOH and cutinase pretreated membranes, respectively). FTIR analysis of modified PLA membranes showed new peaks attributed to human serum albumin (amide bond, NH2 and side chain stretching) appearing within 3600-3000 cm 1 and 1700-1500 cm 1 (Fig. 3). MTT studies also showed that osteoblasts-like and MC-3T3-E1 cells viability increased 2.4 times as compared to untreated PLA membranes. The study therefore shows that this strategy of modifying the surfaces of PLA polymers could significantly improve biocompatibility.

Original languageEnglish
Pages (from-to)1399-1404
Number of pages6
JournalReactive and Functional Polymers
Volume73
Issue number10
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Bioactive
  • Cell viability
  • Cytocompatibility
  • Functionalization
  • Human serum
  • Polylactic acid membranes

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