Biobutanol production from cocoa pod husk through a sequential green method: Depectination, delignification, enzymatic hydrolysis, and extractive fermentation

Maktum Muharja*, Rizki Fitria Darmayanti, Boy Arief Fachri, Bekti Palupi, Istiqomah Rahmawati, Meta Fitri Rizkiana, Helda Wika Amini, Ditta Kharisma Yolanda Putri, Felix Arie Setiawan, Mochamad Asrofi, Arief Widjaja, Abdul Halim

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The effect of various treatments on the production of reducing sugar and biobutanol from cocoa pod husk (CPH) through depectination, delignification, enzymatic hydrolysis, and fermentation was studied to obtain the best strategy for sequential processes to valorize CPH to biobutanol. Surfactants of Tween 80, Polyethylene Glycol (PEG) 6000, and Sodium Dodecyl Sulfate (SDS) was added to enhance the performance of enzymatic hydrolysis, and L-cysteine addition to support fermentation by Clostridium saccharoperbutylacetonicum N1–4. The optimum activity of the cellulase was at 0.25 FPU/g. Depectination, microwave-assisted delignification, and 2.5 g/L PEG 6000 addition sequence produced the highest sugar concentration of 9.24 g/L. The highest butanol and acetone-butanol-ethanol (ABE) concentrations of 20.4 g/L and 54.4 g/L, respectively, were obtained using immobilized cells and L-cysteine supplementation. ABE production by extractive fermentation was enhanced using immobilized cells and L-cysteine supplementation.

Original languageEnglish
Article number101298
JournalBioresource Technology Reports
Volume21
DOIs
Publication statusPublished - Feb 2023
Externally publishedYes

Keywords

  • Biobutanol
  • Cocoa pod husk
  • Depectination
  • Enzymatic hydrolysis
  • Fermentation

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