Biochemical Screening and EPS Production Test of Newly Isolated CaCO3 Precipitating Bacteria as Anti-Corrosion Agent

Muhammad Andry Prio Utomo; Muhammad Mauludi Zulkifli; Kennis Rozana; Nuraini Kartikasari; Sitoresmi Prabaningtyas; Agung Witjoro; Enny Zulaika

doi:10.1063/5.0234861

Biochemical Screening and EPS Production Test of Newly Isolated CaCO₃ Precipitating Bacteria as Anti-Corrosion Agent

Muhammad Andry Prio Utomo^*
, Muhammad Mauludi Zulkifli
, Kennis Rozana
, Nuraini Kartikasari
, Sitoresmi Prabaningtyas
, Agung Witjoro
, Enny Zulaika

^*Corresponding author for this work

State University of Malang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Corrosion is defined as the destruction of a material from its constituent atoms due to surrounding biochemical reactions, which decrease the quality of steel so that it becomes damaged quickly. Steel is one of the most widely used structural materials in coastal areas such as in shipbuilding, submarine pipelines, power plants, and docks. Corrosion has negative impacts on infrastructure, energy, and the economy. This study aims to determine the biochemical character and the potential of EPS production from CaCO3 precipitating isolates from the Southern Coast of Malang. The evaluated biochemical test conducted in our study are cell shape, Gram reaction, oxygen demand test, EPS production test, and antibiotic resistance test. Gram stain test indicates all bacteria are Gram-negative bacteria. CaCO3 precipitating bacteria based on oxygen demand test were divided into two groups: obligate aerobes and facultative anaerobes. All isolates have an inhibition zone and are categorized as sensitive after antibiotics resistance test using ampicillin and chloramphenicol. EPS production test showed only isolates WP2 A3 and WP2 A5 that could produce EPS above 3 mm.

Original language	English
Title of host publication	AIP Conference Proceedings
Editors	Ahmad Taufiq, Hendra Susanto, Chuang-Rung Chang, I. Ching Wang, Hadi Nur, Muhammad Aziz, Sri Rahayu Lestari, Markus Diantoro, Nandang Mufti, Nik Ahmad Nizam Nik Malek, Sunaryono, Siti Zubaidah, Aulanni'am, Indra Wibowo, Adeodatus Yuda Handaya
Publisher	American Institute of Physics
Edition	1
ISBN (Electronic)	9780735451025
DOIs	https://doi.org/10.1063/5.0234861
Publication status	Published - 30 Jan 2025
Event	5th International Conference on Life Science and Technology, ICoLiST 2022 - Hybrid, Malang, Indonesia Duration: 30 Aug 2022 → 31 Aug 2022

Publication series

Name	AIP Conference Proceedings
Number	1
Volume	3186
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	5th International Conference on Life Science and Technology, ICoLiST 2022
Country/Territory	Indonesia
City	Hybrid, Malang
Period	30/08/22 → 31/08/22

Access to Document

10.1063/5.0234861

Cite this

Utomo, M. A. P., Zulkifli, M. M., Rozana, K., Kartikasari, N., Prabaningtyas, S., Witjoro, A., & Zulaika, E. (2025). Biochemical Screening and EPS Production Test of Newly Isolated CaCO₃ Precipitating Bacteria as Anti-Corrosion Agent. In A. Taufiq, H. Susanto, C.-R. Chang, I. C. Wang, H. Nur, M. Aziz, S. R. Lestari, M. Diantoro, N. Mufti, N. A. N. N. Malek, Sunaryono, S. Zubaidah, Aulanni'am, I. Wibowo, & A. Y. Handaya (Eds.), AIP Conference Proceedings (1 ed.). Article 020038 (AIP Conference Proceedings; Vol. 3186, No. 1). American Institute of Physics. https://doi.org/10.1063/5.0234861

Utomo, Muhammad Andry Prio ; Zulkifli, Muhammad Mauludi ; Rozana, Kennis et al. / Biochemical Screening and EPS Production Test of Newly Isolated CaCO₃ Precipitating Bacteria as Anti-Corrosion Agent. AIP Conference Proceedings. editor / Ahmad Taufiq ; Hendra Susanto ; Chuang-Rung Chang ; I. Ching Wang ; Hadi Nur ; Muhammad Aziz ; Sri Rahayu Lestari ; Markus Diantoro ; Nandang Mufti ; Nik Ahmad Nizam Nik Malek ; Sunaryono ; Siti Zubaidah ; Aulanni'am ; Indra Wibowo ; Adeodatus Yuda Handaya. 1. ed. American Institute of Physics, 2025. (AIP Conference Proceedings; 1).

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title = "Biochemical Screening and EPS Production Test of Newly Isolated CaCO3 Precipitating Bacteria as Anti-Corrosion Agent",

abstract = "Corrosion is defined as the destruction of a material from its constituent atoms due to surrounding biochemical reactions, which decrease the quality of steel so that it becomes damaged quickly. Steel is one of the most widely used structural materials in coastal areas such as in shipbuilding, submarine pipelines, power plants, and docks. Corrosion has negative impacts on infrastructure, energy, and the economy. This study aims to determine the biochemical character and the potential of EPS production from CaCO3 precipitating isolates from the Southern Coast of Malang. The evaluated biochemical test conducted in our study are cell shape, Gram reaction, oxygen demand test, EPS production test, and antibiotic resistance test. Gram stain test indicates all bacteria are Gram-negative bacteria. CaCO3 precipitating bacteria based on oxygen demand test were divided into two groups: obligate aerobes and facultative anaerobes. All isolates have an inhibition zone and are categorized as sensitive after antibiotics resistance test using ampicillin and chloramphenicol. EPS production test showed only isolates WP2 A3 and WP2 A5 that could produce EPS above 3 mm.",

author = "Utomo, \{Muhammad Andry Prio\} and Zulkifli, \{Muhammad Mauludi\} and Kennis Rozana and Nuraini Kartikasari and Sitoresmi Prabaningtyas and Agung Witjoro and Enny Zulaika",

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Utomo, MAP, Zulkifli, MM, Rozana, K, Kartikasari, N, Prabaningtyas, S, Witjoro, A & Zulaika, E 2025, Biochemical Screening and EPS Production Test of Newly Isolated CaCO₃ Precipitating Bacteria as Anti-Corrosion Agent. in A Taufiq, H Susanto, C-R Chang, IC Wang, H Nur, M Aziz, SR Lestari, M Diantoro, N Mufti, NANN Malek, Sunaryono, S Zubaidah, Aulanni'am, I Wibowo & AY Handaya (eds), AIP Conference Proceedings. 1 edn, 020038, AIP Conference Proceedings, no. 1, vol. 3186, American Institute of Physics, 5th International Conference on Life Science and Technology, ICoLiST 2022, Hybrid, Malang, Indonesia, 30/08/22. https://doi.org/10.1063/5.0234861

Biochemical Screening and EPS Production Test of Newly Isolated CaCO₃ Precipitating Bacteria as Anti-Corrosion Agent. / Utomo, Muhammad Andry Prio; Zulkifli, Muhammad Mauludi; Rozana, Kennis et al.
AIP Conference Proceedings. ed. / Ahmad Taufiq; Hendra Susanto; Chuang-Rung Chang; I. Ching Wang; Hadi Nur; Muhammad Aziz; Sri Rahayu Lestari; Markus Diantoro; Nandang Mufti; Nik Ahmad Nizam Nik Malek; Sunaryono; Siti Zubaidah; Aulanni'am; Indra Wibowo; Adeodatus Yuda Handaya. 1. ed. American Institute of Physics, 2025. 020038 (AIP Conference Proceedings; Vol. 3186, No. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Biochemical Screening and EPS Production Test of Newly Isolated CaCO3 Precipitating Bacteria as Anti-Corrosion Agent

AU - Utomo, Muhammad Andry Prio

AU - Zulkifli, Muhammad Mauludi

AU - Rozana, Kennis

AU - Kartikasari, Nuraini

AU - Prabaningtyas, Sitoresmi

AU - Witjoro, Agung

AU - Zulaika, Enny

PY - 2025/1/30

Y1 - 2025/1/30

N2 - Corrosion is defined as the destruction of a material from its constituent atoms due to surrounding biochemical reactions, which decrease the quality of steel so that it becomes damaged quickly. Steel is one of the most widely used structural materials in coastal areas such as in shipbuilding, submarine pipelines, power plants, and docks. Corrosion has negative impacts on infrastructure, energy, and the economy. This study aims to determine the biochemical character and the potential of EPS production from CaCO3 precipitating isolates from the Southern Coast of Malang. The evaluated biochemical test conducted in our study are cell shape, Gram reaction, oxygen demand test, EPS production test, and antibiotic resistance test. Gram stain test indicates all bacteria are Gram-negative bacteria. CaCO3 precipitating bacteria based on oxygen demand test were divided into two groups: obligate aerobes and facultative anaerobes. All isolates have an inhibition zone and are categorized as sensitive after antibiotics resistance test using ampicillin and chloramphenicol. EPS production test showed only isolates WP2 A3 and WP2 A5 that could produce EPS above 3 mm.

AB - Corrosion is defined as the destruction of a material from its constituent atoms due to surrounding biochemical reactions, which decrease the quality of steel so that it becomes damaged quickly. Steel is one of the most widely used structural materials in coastal areas such as in shipbuilding, submarine pipelines, power plants, and docks. Corrosion has negative impacts on infrastructure, energy, and the economy. This study aims to determine the biochemical character and the potential of EPS production from CaCO3 precipitating isolates from the Southern Coast of Malang. The evaluated biochemical test conducted in our study are cell shape, Gram reaction, oxygen demand test, EPS production test, and antibiotic resistance test. Gram stain test indicates all bacteria are Gram-negative bacteria. CaCO3 precipitating bacteria based on oxygen demand test were divided into two groups: obligate aerobes and facultative anaerobes. All isolates have an inhibition zone and are categorized as sensitive after antibiotics resistance test using ampicillin and chloramphenicol. EPS production test showed only isolates WP2 A3 and WP2 A5 that could produce EPS above 3 mm.

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DO - 10.1063/5.0234861

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A2 - Wang, I. Ching

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A2 - Lestari, Sri Rahayu

A2 - Diantoro, Markus

A2 - Mufti, Nandang

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A2 - Zubaidah, Siti

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Utomo MAP, Zulkifli MM, Rozana K, Kartikasari N, Prabaningtyas S, Witjoro A et al. Biochemical Screening and EPS Production Test of Newly Isolated CaCO₃ Precipitating Bacteria as Anti-Corrosion Agent. In Taufiq A, Susanto H, Chang CR, Wang IC, Nur H, Aziz M, Lestari SR, Diantoro M, Mufti N, Malek NANN, Sunaryono, Zubaidah S, Aulanni'am, Wibowo I, Handaya AY, editors, AIP Conference Proceedings. 1 ed. American Institute of Physics. 2025. 020038. (AIP Conference Proceedings; 1). doi: 10.1063/5.0234861