Biodecolorization of methyl orange by mixed cultures of brown-rot fungus daedalea dickinsii and bacterium pseudomonas aeruginosa

This study investigated on the decolorization of methyl orange (MO) by mixed cultures of brown-rot fungus (BRF) Daedalea dickinsii and bacterium Pseudomonas aeruginosa. P. aeruginosa was added into D. dickinsii culture at 2, 4, 6, 8, 10 mL (1 mL = 5.05 × 10¹² CFU). All of mixed cultures had ability to decolorize MO (final concentration 100 mg/L) in potato dextrose broth (PDB) medium for 7 days incubation. The addition of 4 mL of P. aeruginosa showed the highest MO biodecolorization approximately 97,99%, while by D. dickinsii only was 67,54%. C₁₅H₁₉N₃O₅S; C₁₆H₂₁N₃O₅S; C₁₇H₂₃N₃O₆S; and C₁₅H₁₉N₃O₆S were identified as MO metabolites. This study indicated that mixed cultures of D. dickinsii and P. aeruginosa have great potential for high efficiency, fast and cheap dye wastewater treatment.