Biodegradation of crude oil by Ralstonia pickettii under high salinity medium

Adi Setyo Purnomo*, Hamdan Dwi Rizqi, Lia Harmelia, Silmi Diah Anggraeni, Ranny Etna Melati, Zulfah Hernida Damayanti, Ova Mar atus Shafwah, Fransisca Cahyaning Kusuma

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Bacterium Ralstonia pickettii has ability to survive and thrive in low nutrient condition as well as a capability to remediate some pollutants and using them as carbon and energy source. In this study,the ability of R. pickettii on biodegradation of crude oil under high salinity medium was investigated. R. pickettii was pre-incubated in nutrient broth (NB) medium and then, washed and transferred to artificial seawater medium. Crude oil was added to each culture and incubated for 7 and 14 days. The biodegradation of crude oil was analysed using Gas chromatography mass spectrometry (GC-MS). The result showed that R. pickettii had successfully degraded the crude oil in the high salinity artificial seawater. The incubation on 7 and 14 days did not show a significant effect on the number of the degraded compounds. The optimum recovery percent was obtained from the derivation of 2,6,10,14-tetramethyl hexadecane with the recovery percentage of 12.7% and 16.0% for 7 and 14 days respectively. This study indicates that R. picketti can be potentially used for bioremediation of crude oil under high salinity environments.

Original languageEnglish
Pages (from-to)377-380
Number of pages4
JournalMalaysian Journal of Fundamental and Applied Sciences
Volume15
Issue number3
DOIs
Publication statusPublished - May 2019

Keywords

  • Biodegradation
  • Ralstonia pickettii
  • crude oil
  • high salinity medium

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