Biodelignification of Sengon Wood Using Trametes Versicolor

Delignification is a process of changing the chemical structure of lignocellulosic biomass with the aim of selectively degrading lignin, by break down the chemical bonds of lignin with other chemical components of lignocellulosic materials (cellulose and hemicellulose). Biological pretreatment is a one of many pretreatments that can be used because of its environmentally friendly nature. White rot fungi can degrade lignin seems promising because they consume less energy and cause less environmental damage. The purpose of this study was to determine the effect of variations in bio-delignification time using Trametes versicolor on the lignin content of sengon wood. The bio-delignification process was carried out by soaking sengon wood powder (40 mesh) with distilled water for 24 hours. The wet wood was sterilized by pressure cooker in 30 minutes. After that, the samples were inoculated with 50 ml of suspension of Trametes versicolor in potatoes dextrose broth media that had been grown previously for 7 days. The samples were then incubated at room temperature for various incubation time of (10-50 days). From the result, the lignin content of sengon wood decreased and the cellulose and hemicellulose content increased by increasing incubation time. The lowest levels of lignin were were achieved at the longest incubation for 50 days (3.18%). Then seen from the SEM results of sengon wood powder with a biodelignification time of 50 days there were tears in the outer layer.