Biodiesel production from rice bran by a two-step in-situ process

Pei Jing Shiu, Setiyo Gunawan, Wen Hao Hsieh, Novy S. Kasim, Yi Hsu Ju*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)


The production of fatty acid methyl esters (FAMEs) by a two-step in-situ transesterification from two kinds of rice bran was investigated in this study. The method included an in-situ acid-catalyzed esterification followed by an in-situ base-catalyzed transesterification. Free fatty acids (FFAs) level was reduced to less than 1% for both rice bran A (initial FFAs content = 3%) and rice bran B (initial FFAs content = 30%) in the first step under the following conditions: 10 g rice bran, methanol to rice bran ratio 15 mL/g, H2SO4 to rice bran mass ratio 0.18, 60 °C reaction temperature, 600 rpm stirring rate, 15 min reaction time. The organic phase of the first step product was collected and subjected to a second step reaction by adding 8 mL of 5 N NaOH solution and allowing to react for 60 and 30 min for rice bran A and rice bran B, respectively. FAMEs yields of 96.8% and 97.4% were obtained for rice bran A and rice bran B, respectively, after this two-step in-situ reaction.

Original languageEnglish
Pages (from-to)984-989
Number of pages6
JournalBioresource Technology
Issue number3
Publication statusPublished - Feb 2010


  • Rice bran
  • Two-step in-situ transesterification


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