Bioprospecting of parijoto fruit extract (medinilla speciosa) as antioxidant and immunostimulant: Phagocytosis activity of macrophage cells

Noor Nailis Sa’adah*, Adik Mayang Indiani, Awik Puji Dyah Nurhayati, Nova Maulidina Ashuri

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Citations (Scopus)

Abstract

Parijoto (Medinilla speciosa) is one of fruits in Indonesia that contains high anthocyanin, which is a flavonoid compound that exerts beneficial effects on blood pressure, endothelial function, oxidative stress and as antioxidants. Antioxidants helps in converting the radicals to less reactive species, or as an inhibitor of the process of oxidation. Antioxidants also can help the body's defense system if there are elements of disease generation. Compounds that can increase the body's resistance to various infections through increasing of the oxidative activity of neutrophils, phagocytic cell activity and stimulating cytotoxic cells are called immunostimulants. This study aimed to determine the antioxidant activity and phagocytosis of macrophage cells that were given methanol extract of parijoto pericarp and whole fruit (M. speciosa). Antioxidant activity of parijoto pericarp and whole fruit (M. speciosa) were calculated using DPPH test. Phagocytosis activity test was carried out in 5 groups, control; macrophage cells were given 125 ppm; 250 ppm; 500 ppm; and 1000 ppm of parijoto pericarp and whole fruit extract. Macrophage cells were isolated from the peritoneum part of mice, then cultured using RPMI medium for 24 hours. Non-specific phagocytosis ability tests were carried out in vitro using latex beads. The result showed that the total antioxidant activity of methanol extract of parijoto (M. speciosa) pericarp and whole fruit were very strong with IC50 values of 29.68 ppm and 23.73 ppm respectively. Administration of methanol extract of parijoto (M. speciosa) pericarp and whole fruit increased phagocytosis capacity (highest at 1000 µg / ml with a value of 61.50% and 74.00%). Phagocytosis index values also increased, where the highest increase at concentrations of 500 µg / ml with values of 2.53 and 3.16.

Original languageEnglish
Title of host publication6th International Conference on Biological Science, ICBS 2019
Subtitle of host publication"Biodiversity as a Cornerstone for Embracing Future Humanity"
EditorsHendri Trisakti S.G. Saragih, Miftahul Ilmi, Sri Nopitasari, Liya Audinah, Annisaa Widyasari, Mutiara Arum Sari, Medina Masri, Prayolga Toban Palilu, Beni Hendro Prabowo, Siti Soleha, Annisa Solikhah, Nita Fitriana
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735420205
DOIs
Publication statusPublished - 16 Sept 2020
Event6th International Conference on Biological Science: Biodiversity as a Cornerstone for Embracing Future Humanity, ICBS 2019 - Yogyakarta, Indonesia
Duration: 10 Oct 201911 Oct 2019

Publication series

NameAIP Conference Proceedings
Volume2260
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference6th International Conference on Biological Science: Biodiversity as a Cornerstone for Embracing Future Humanity, ICBS 2019
Country/TerritoryIndonesia
CityYogyakarta
Period10/10/1911/10/19

Fingerprint

Dive into the research topics of 'Bioprospecting of parijoto fruit extract (medinilla speciosa) as antioxidant and immunostimulant: Phagocytosis activity of macrophage cells'. Together they form a unique fingerprint.

Cite this