Cellulase activity of cellulolytic bacteria isolated from palangkaraya peat, central kalimantan

Peat soil has the highest content of organic matter with cellulose. Cellulose decomposition can be carried out by cellulolytic bacteria which are capable of producing cellulase. Isolates D1, D2, D3, U2, U3 and U4 are indigenous cellulolytic bacteria from peat Palangkaraya, Kalimantan and selected to produce cellulase in a selective medium of Carboxy Methyl Cellulose. The research objective was to determine the effect of pH, temperature and incubation time on cellulase activity from indigenous isolates. Cellulase activity was measured using spectrophotometric method with dinitrosalyclic acid (DNS) and glucose standard curve. The optimum pH for cellulase activity of the six isolates occurred at pH 5 with the highest enzyme activity of 997.4 U/ml in U4 isolate. The highest temperature for cellulase activity is 30°C for D3 isolates with 1746.1 U/ml and 40°C for D2 isolates with 1718.7 U/ml. While the incubation time required 12 hours to get the highest cellulase activity was in U2 isolates of 957.7 U/ml.