TY - JOUR
T1 - Cellulose oxidation and bleaching processes based on recombinant Myriococcum thermophilum cellobiose dehydrogenase
AU - Flitsch, Annemarie
AU - Prasetyo, Endry Nugroho
AU - Sygmund, Christoph
AU - Ludwig, Roland
AU - Nyanhongo, Gibson S.
AU - Guebitz, Georg M.
N1 - Funding Information:
The authors are grateful for the financial support from European Union Cotton Bleach Project .
PY - 2013/1/10
Y1 - 2013/1/10
N2 - Myriococcum thermophilum cellobiose dehydrogenase (MtCDH) was expressed in Pichia pastoris using the pPICZαA expression vector under the control of methanol inducible AOX promoter. The purified recombinant MtCDH with a specific activity of 3.1Umg-1 was characterized to obtain kinetic constants for various carbohydrate substrates. Additionally, the C1 oxidation of the reducing ends of cellobiose, cellotetraose and maltotriose by MtCDH was verified by HPLC-MS. MtCDH was employed to oxidize several different cellulose-based materials by production of hydrogen peroxide. Based on the obtained results a one-pot enzymatic scouring/bleaching process for cotton fabrics was developed using pectinases as scouring agent and MtCDH to produce H2O2 for bleaching. An average increase in whiteness (Berger) ΔE of 26 and an average 95% increase in wettability were observed in all MtCDH treated fabrics. In addition, MtCDH oxidized typical colored cotton flavonoids (morin, rutin, isoquercitrin).
AB - Myriococcum thermophilum cellobiose dehydrogenase (MtCDH) was expressed in Pichia pastoris using the pPICZαA expression vector under the control of methanol inducible AOX promoter. The purified recombinant MtCDH with a specific activity of 3.1Umg-1 was characterized to obtain kinetic constants for various carbohydrate substrates. Additionally, the C1 oxidation of the reducing ends of cellobiose, cellotetraose and maltotriose by MtCDH was verified by HPLC-MS. MtCDH was employed to oxidize several different cellulose-based materials by production of hydrogen peroxide. Based on the obtained results a one-pot enzymatic scouring/bleaching process for cotton fabrics was developed using pectinases as scouring agent and MtCDH to produce H2O2 for bleaching. An average increase in whiteness (Berger) ΔE of 26 and an average 95% increase in wettability were observed in all MtCDH treated fabrics. In addition, MtCDH oxidized typical colored cotton flavonoids (morin, rutin, isoquercitrin).
KW - Bleaching
KW - Cellobiose dehydrogenase
KW - Cotton
KW - Recombinant DNA
UR - http://www.scopus.com/inward/record.url?scp=84870364865&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2012.10.007
DO - 10.1016/j.enzmictec.2012.10.007
M3 - Article
C2 - 23199740
AN - SCOPUS:84870364865
SN - 0141-0229
VL - 52
SP - 60
EP - 67
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 1
ER -