Characterization of diffuse reflectance spectrum from vegetable leaves as the techniques of chlorophyll concentration measurement

Wilda Prihasty, Aulia M.T. Nasution, Sekartedjo Koentjoro, Iwan Cony Setiadi

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Chlorophyll is a main biochemistry component for photosynthesis and a health indicator for plant. Chlorophyll concentration can be measured by non-destructive and non-contact method using Diffuse Reflectance Spectroscopy. Phantom consist of intralipid, aquades, gelatin and chlorophyll. Chlorophyll with certain concentration that known by absorption technique is used to be a primary stage for DRS. Non linear least square data fitting using diffuse reflectance mathematical model is used to determine parameters from phantom. The value of parameters is used for chlorophyll concentration measurement and will be compared with the chlorophyll concentration from absorption technique. When the system have been quantified well, the system will be used for chlorophyll concentration measurement for leaves without a complicated sample preparation such as absorption technique. Phantom reflectance spectroscopy has been tested and has a similar spectrum shape with leave's reflectance. Chlorophyll concentration measurement has been tested from three species of vegetables with three variation concentration and got error value for mustard, spinach and kale respectively 3%-10%; 2.5%-10% and 16%-31%.

Original languageEnglish
Title of host publicationThird International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2018
EditorsAulia Nasution, Agus Muhammad Hatta
PublisherSPIE
ISBN (Electronic)9781510627543
DOIs
Publication statusPublished - 2019
Event3rd International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2018 - Surabaya, Indonesia
Duration: 1 Aug 20182 Aug 2018

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11044
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

Conference3rd International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2018
Country/TerritoryIndonesia
CitySurabaya
Period1/08/182/08/18

Keywords

  • Chlorophyll Concentration
  • Diffuse Reflectance Spectroscopy
  • Fitting
  • Phantom

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