Characterization of mannanase isolated from corncob waste bacteria

S. Wahyuni*, A. Khaeruni, A. S. Purnomo, Asranudin, Holilah, Fatahu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Mannanase bacteria has been isolated from some sources such as oil waste, activated sludge, decayed konjac, soil and sagoo hump, which mannanase enzyme has been isolated from bacteria, fungi and some fruits. This study focused on the characterization of mannanase bacteria isolated from corncobs waste. Nine isolates bacteria were successfully screened, which 2 isolates showed the greatest mannolitic index i.e., IB.1.6 and IB.1.10. The character of both isolates were Gram-negative and rod cells. Characteristics physiology of IB.1.6 and IB.1.10 isolates showed positive result on the fermentation of mannose, rhamnose, lactose and glucose, as well as utilization of citrate and catalase. Mannanase from IB.1.6 and IB.1.10 isolates were stable at wide temperature range 30-90 °C and pH range 5-8, which temperature and pH optimum of 60 and 8 °C, respectively. Type of metal ion (Co2+ and Cu2+) and surfactants (Tween 20 and Tween 80) affected the activity of 2 enzymes.

Original languageEnglish
Pages (from-to)1119-1120
Number of pages2
JournalAsian Journal of Chemistry
Volume29
Issue number5
DOIs
Publication statusPublished - 2017

Keywords

  • Bacteria
  • Corncob
  • Mannanase
  • Waste

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