TY - JOUR
T1 - Coupling of aromatic amines onto syringylglycerol β-guaiacylether using Bacillus SF spore laccase
T2 - A model for functionalization of lignin-based materials
AU - Kudanga, Tukayi
AU - Nugroho Prasetyo, Endry
AU - Sipilä, Jussi
AU - Eberl, Anita
AU - Nyanhongo, Gibson S.
AU - Guebitz, Georg M.
N1 - Funding Information:
Acknowledgment for support of this work is made to Austrian Academic Exchange Service (ÖAD) and European Union Biorenew Project [Sixth Framework Programme (FP6-2004-NMP-NI-4)].
PY - 2009/12
Y1 - 2009/12
N2 - The potential of Bacillus SF spore laccase for coupling aromatic amines to lignin model molecules as a way of creating a stable reactive surface was investigated. The Bacillus spore laccase was shown to be active within the neutral to alkaline conditions (pH 7-8.5) and was more resistant to common laccase inhibitors than fungal laccases. Using this enzyme, tyramine was successfully covalently coupled onto syringylglycerol β-guaiacylether via a 4-O-5 bond, leaving the -NH2 group free for further attachment of functional molecules. This study demonstrates the potential of Bacillus SF spore laccase for application in lignocellulose surface functionalization and other coupling reactions which can be carried out at neutral to alkaline pH under extreme conditions which normally inhibit fungal laccases.
AB - The potential of Bacillus SF spore laccase for coupling aromatic amines to lignin model molecules as a way of creating a stable reactive surface was investigated. The Bacillus spore laccase was shown to be active within the neutral to alkaline conditions (pH 7-8.5) and was more resistant to common laccase inhibitors than fungal laccases. Using this enzyme, tyramine was successfully covalently coupled onto syringylglycerol β-guaiacylether via a 4-O-5 bond, leaving the -NH2 group free for further attachment of functional molecules. This study demonstrates the potential of Bacillus SF spore laccase for application in lignocellulose surface functionalization and other coupling reactions which can be carried out at neutral to alkaline pH under extreme conditions which normally inhibit fungal laccases.
KW - Bacillus SF spore laccase
KW - Coupling
KW - Syringylglycerol β-guaiacylether
UR - http://www.scopus.com/inward/record.url?scp=72049090088&partnerID=8YFLogxK
U2 - 10.1016/j.molcatb.2009.06.003
DO - 10.1016/j.molcatb.2009.06.003
M3 - Article
AN - SCOPUS:72049090088
SN - 1381-1177
VL - 61
SP - 143
EP - 149
JO - Journal of Molecular Catalysis - B Enzymatic
JF - Journal of Molecular Catalysis - B Enzymatic
IS - 3-4
ER -