Curcumin-Loaded Liposome Preparation in Ultrasound Environment under Pressurized Carbon Dioxide

Wahyudiono, Jiayang He, Xin Hu, Siti Machmudah, Keiji Yasuda, Seiichi Takami, Hideki Kanda*, Motonobu Goto

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Curcumin-loaded liposomes were prepared using a supercritical carbon dioxide (SCCO2 )– ultrasound environment system. The experiments were performed at temperatures of 40–70C and pressures of 10–25 MPa in a batch system with ultrasonication for 60 min. Transmission electron microscopy (TEM) images revealed liposome products with spherical morphologies and diameters of <100 nm. Dynamic light scattering (DLS) analysis indicated that the curcumin-loaded liposome nanosuspension exhibited good stability. Changing the operating conditions influenced the amount of liposome-encapsulated curcumin; as the operating temperature or pressure increased, the diameter of the liposome products and the amount of liposome-encapsulated curcumin increased and decreased, respectively. Herein, we described an innovative and practical organic-solvent-free method for generating liposomes from phospholipids.

Original languageEnglish
Article number1469
Issue number10
Publication statusPublished - 1 May 2022


  • curcumin
  • encapsulation
  • liposome
  • phospholipid
  • supercritical


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