Cytotoxic activity of ethanolic extract of the marine sponge Aaptos suberitoides against T47D cell

Awik Puji Dyah Nurhayati; Rarastoeti Prastiwi; Sukardiman; Tri Wahyuningsih

doi:10.1063/1.5030235

Cytotoxic activity of ethanolic extract of the marine sponge Aaptos suberitoides against T47D cell

Awik Puji Dyah Nurhayati^*
, Rarastoeti Prastiwi
, Sukardiman
, Tri Wahyuningsih

^*Corresponding author for this work

Gadjah Mada University
Universitas Airlangga
Institut Teknologi Sepuluh Nopember

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

4 Citations (Scopus)

Abstract

Aaptos suberitoides marine sponge produce many kinds of secondary metabolites. The purpose of this study were to examine the cytotoxic, proliferation inhibition and apoptosis induction of marine sponge A.suberitoides. The sponge was extracted with 96 % ethanol. Ethanol extract cytotoxicity assay were performed with MTT method (Microculture Tetrazolium) against to cell line of T47D. The proliferation inhibition were done by doubling time. The apoptosis induction by observing the treated cell morphology after staining with acrydine orange. The results show that cytotoxic activity of the ethanol extract was 153.109 μg/mL, inhibits cell proliferation cell lines of T47D at 24 hours of incubation and apoptosis induction.

Original language	English
Title of host publication	Proceedings of the 3rd International Conference on Materials and Metallurgical Engineering and Technology, ICOMMET 2017
Subtitle of host publication	Advancing Innovation in Materials Science, Technology and Applications for Sustainable Future
Editors	Mas Irfan P. Hidayat
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735416406
DOIs	https://doi.org/10.1063/1.5030235
Publication status	Published - 3 Apr 2018
Event	3rd International Conference on Materials and Metallurgical Engineering and Technology: Advancing Innovation in Materials Science, Technology and Applications for Sustainable Future, ICOMMET 2017 - Surabaya, Indonesia Duration: 30 Oct 2017 → 31 Oct 2017

Publication series

Name	AIP Conference Proceedings
Volume	1945
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	3rd International Conference on Materials and Metallurgical Engineering and Technology: Advancing Innovation in Materials Science, Technology and Applications for Sustainable Future, ICOMMET 2017
Country/Territory	Indonesia
City	Surabaya
Period	30/10/17 → 31/10/17

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Keywords

Aaptos suberitoides
Cytotoxic Activity
Ethanolic Extract
Marine Sponge
T47D Cell

Access to Document

10.1063/1.5030235

Cite this

Nurhayati, A. P. D., Prastiwi, R., Sukardiman, & Wahyuningsih, T. (2018). Cytotoxic activity of ethanolic extract of the marine sponge Aaptos suberitoides against T47D cell. In M. I. P. Hidayat (Ed.), Proceedings of the 3rd International Conference on Materials and Metallurgical Engineering and Technology, ICOMMET 2017: Advancing Innovation in Materials Science, Technology and Applications for Sustainable Future Article 020013 (AIP Conference Proceedings; Vol. 1945). American Institute of Physics Inc.. https://doi.org/10.1063/1.5030235

Nurhayati, Awik Puji Dyah ; Prastiwi, Rarastoeti ; Sukardiman et al. / Cytotoxic activity of ethanolic extract of the marine sponge Aaptos suberitoides against T47D cell. Proceedings of the 3rd International Conference on Materials and Metallurgical Engineering and Technology, ICOMMET 2017: Advancing Innovation in Materials Science, Technology and Applications for Sustainable Future. editor / Mas Irfan P. Hidayat. American Institute of Physics Inc., 2018. (AIP Conference Proceedings).

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title = "Cytotoxic activity of ethanolic extract of the marine sponge Aaptos suberitoides against T47D cell",

abstract = "Aaptos suberitoides marine sponge produce many kinds of secondary metabolites. The purpose of this study were to examine the cytotoxic, proliferation inhibition and apoptosis induction of marine sponge A.suberitoides. The sponge was extracted with 96 \% ethanol. Ethanol extract cytotoxicity assay were performed with MTT method (Microculture Tetrazolium) against to cell line of T47D. The proliferation inhibition were done by doubling time. The apoptosis induction by observing the treated cell morphology after staining with acrydine orange. The results show that cytotoxic activity of the ethanol extract was 153.109 μg/mL, inhibits cell proliferation cell lines of T47D at 24 hours of incubation and apoptosis induction.",

keywords = "Aaptos suberitoides, Cytotoxic Activity, Ethanolic Extract, Marine Sponge, T47D Cell",

author = "Nurhayati, \{Awik Puji Dyah\} and Rarastoeti Prastiwi and Sukardiman and Tri Wahyuningsih",

note = "Publisher Copyright: {\textcopyright} 2018 Author(s).; 3rd International Conference on Materials and Metallurgical Engineering and Technology: Advancing Innovation in Materials Science, Technology and Applications for Sustainable Future, ICOMMET 2017 ; Conference date: 30-10-2017 Through 31-10-2017",

year = "2018",

month = apr,

day = "3",

doi = "10.1063/1.5030235",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

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Nurhayati, APD, Prastiwi, R, Sukardiman & Wahyuningsih, T 2018, Cytotoxic activity of ethanolic extract of the marine sponge Aaptos suberitoides against T47D cell. in MIP Hidayat (ed.), Proceedings of the 3rd International Conference on Materials and Metallurgical Engineering and Technology, ICOMMET 2017: Advancing Innovation in Materials Science, Technology and Applications for Sustainable Future., 020013, AIP Conference Proceedings, vol. 1945, American Institute of Physics Inc., 3rd International Conference on Materials and Metallurgical Engineering and Technology: Advancing Innovation in Materials Science, Technology and Applications for Sustainable Future, ICOMMET 2017, Surabaya, Indonesia, 30/10/17. https://doi.org/10.1063/1.5030235

Cytotoxic activity of ethanolic extract of the marine sponge Aaptos suberitoides against T47D cell. / Nurhayati, Awik Puji Dyah; Prastiwi, Rarastoeti; Sukardiman et al.
Proceedings of the 3rd International Conference on Materials and Metallurgical Engineering and Technology, ICOMMET 2017: Advancing Innovation in Materials Science, Technology and Applications for Sustainable Future. ed. / Mas Irfan P. Hidayat. American Institute of Physics Inc., 2018. 020013 (AIP Conference Proceedings; Vol. 1945).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Cytotoxic activity of ethanolic extract of the marine sponge Aaptos suberitoides against T47D cell

AU - Nurhayati, Awik Puji Dyah

AU - Prastiwi, Rarastoeti

AU - Sukardiman,

AU - Wahyuningsih, Tri

PY - 2018/4/3

Y1 - 2018/4/3

N2 - Aaptos suberitoides marine sponge produce many kinds of secondary metabolites. The purpose of this study were to examine the cytotoxic, proliferation inhibition and apoptosis induction of marine sponge A.suberitoides. The sponge was extracted with 96 % ethanol. Ethanol extract cytotoxicity assay were performed with MTT method (Microculture Tetrazolium) against to cell line of T47D. The proliferation inhibition were done by doubling time. The apoptosis induction by observing the treated cell morphology after staining with acrydine orange. The results show that cytotoxic activity of the ethanol extract was 153.109 μg/mL, inhibits cell proliferation cell lines of T47D at 24 hours of incubation and apoptosis induction.

AB - Aaptos suberitoides marine sponge produce many kinds of secondary metabolites. The purpose of this study were to examine the cytotoxic, proliferation inhibition and apoptosis induction of marine sponge A.suberitoides. The sponge was extracted with 96 % ethanol. Ethanol extract cytotoxicity assay were performed with MTT method (Microculture Tetrazolium) against to cell line of T47D. The proliferation inhibition were done by doubling time. The apoptosis induction by observing the treated cell morphology after staining with acrydine orange. The results show that cytotoxic activity of the ethanol extract was 153.109 μg/mL, inhibits cell proliferation cell lines of T47D at 24 hours of incubation and apoptosis induction.

KW - Aaptos suberitoides

KW - Cytotoxic Activity

KW - Ethanolic Extract

KW - Marine Sponge

KW - T47D Cell

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DO - 10.1063/1.5030235

M3 - Conference contribution

AN - SCOPUS:85045623892

T3 - AIP Conference Proceedings

BT - Proceedings of the 3rd International Conference on Materials and Metallurgical Engineering and Technology, ICOMMET 2017

A2 - Hidayat, Mas Irfan P.

PB - American Institute of Physics Inc.

T2 - 3rd International Conference on Materials and Metallurgical Engineering and Technology: Advancing Innovation in Materials Science, Technology and Applications for Sustainable Future, ICOMMET 2017

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ER -

Nurhayati APD, Prastiwi R, Sukardiman, Wahyuningsih T. Cytotoxic activity of ethanolic extract of the marine sponge Aaptos suberitoides against T47D cell. In Hidayat MIP, editor, Proceedings of the 3rd International Conference on Materials and Metallurgical Engineering and Technology, ICOMMET 2017: Advancing Innovation in Materials Science, Technology and Applications for Sustainable Future. American Institute of Physics Inc. 2018. 020013. (AIP Conference Proceedings). doi: 10.1063/1.5030235

Cytotoxic activity of ethanolic extract of the marine sponge Aaptos suberitoides against T47D cell

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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