In the present work, a multi-gender bike, which can be used by men and women, was designed. The integrated design method is used to develop a bicycle frame. As a human transportation the frame is must be light, strong, and comfortable for rider. The frame of bicycle is built with the material of aluminum alloy T-6061 and is produced as prototype for a multi gender purpose. With 100 kg load the strength of material is calculated, and the result shows that the frame critical strength is smaller than material ultimate strength. The prototype was tested by 5 respondents who have an average weight of 68.2 kg, and height 169.4 cm. Pedal test is conducted with 5 level of speed such as 6, 8, 10, 12, and 14 mph in 6 minutes of cycling respectively. The paddle energy during cycling was determined. The results show that the paddle energy increase and follow the increasing of speed. However since 10 mph of speed the paddle energy tends to be fixed with average value of 40 kcal. Therefore the tension leg muscles before and after cycling is going together by increasing the speed. The RULA method is used for determining the ergonomic of multi-gender bicycle, and the result shows that the value of risk injury is 3, that mean the developed bike is ergonomic.