TY - GEN
T1 - Determination of Rhodamine-B and Amaranth in snacks at primary school Sukolilo district of Surabaya-Indonesia by thin layer chromatography
AU - Tonica, Widya Wahyu
AU - Hardianti, Maretna Fitri
AU - Prasetya, Surya Aji
AU - Rachmaniah, Orchidea
N1 - Publisher Copyright:
© 2018 Author(s).
PY - 2018/12/14
Y1 - 2018/12/14
N2 - Rhodamine B and Amaranth, banned artificial food red dyes, were determined from the primary school's food stall snacks such as sausage, beefburger, and tomato sauce. Both of them are commonly used for coloring textiles, paper, soap, leather, drugs, etc. Hence, they are toxic and carcinogenic for human. Unfortunately, they can still be found in children's snacks due to the exciting stability of color and low price. For that reason, Rhodamine Β and Amaranth were tested from children's snacks at Sukolilo District with huge number of primary schools ca. 34 private and state primary schools. Initially, a survey was conducted; revealing the presence of the food stall at the studied primary schools and identifying kind of snacks. Only sausage, beef burger, and tomato sauce were sampled because of containing red color and favorites amongst the snacks. In case of dyes extraction, ethanol (70 v/v) was used as a solvent combining with vortex and centrifugation method. Moreover, ethanol (EtOH): ammonia (19:1, v/v/v/v); butanol: EtOH: H2O: Ammonia (50:25:25:10, v/v/v/v); 1,2 propane-diol: Ammonia (4:2, v/v); EtOH: Ammonia (9:2, v/v), and EtOH: Ammonia (8:4, v/v) were optimized as a mobile phase. Revealing that EtOH: Ammonia (9:2, v/v) is the most appropriate mobile phase for qualitatively separating of Rhodamine B, Amaranth, Red Allure, Erythrosine, and Ponceau 4R. The result showed that 51% of elementary schools at Sukolilo district, Surabaya allow the food stall presence around the school area, while the remaining not. None of the samples contain Rhodamine B. While amaranth is found in some of the comparable ketchup products. Therefore, periodical inspection by an authorized body is needed to keep the producer, i.e. food industry, strictly fit the regulations. In case of red dyes, Erythrosine is the mostly used in the ketchup, while Ponceau 4R and red allure are only used in some of the ketchup and corned beef.
AB - Rhodamine B and Amaranth, banned artificial food red dyes, were determined from the primary school's food stall snacks such as sausage, beefburger, and tomato sauce. Both of them are commonly used for coloring textiles, paper, soap, leather, drugs, etc. Hence, they are toxic and carcinogenic for human. Unfortunately, they can still be found in children's snacks due to the exciting stability of color and low price. For that reason, Rhodamine Β and Amaranth were tested from children's snacks at Sukolilo District with huge number of primary schools ca. 34 private and state primary schools. Initially, a survey was conducted; revealing the presence of the food stall at the studied primary schools and identifying kind of snacks. Only sausage, beef burger, and tomato sauce were sampled because of containing red color and favorites amongst the snacks. In case of dyes extraction, ethanol (70 v/v) was used as a solvent combining with vortex and centrifugation method. Moreover, ethanol (EtOH): ammonia (19:1, v/v/v/v); butanol: EtOH: H2O: Ammonia (50:25:25:10, v/v/v/v); 1,2 propane-diol: Ammonia (4:2, v/v); EtOH: Ammonia (9:2, v/v), and EtOH: Ammonia (8:4, v/v) were optimized as a mobile phase. Revealing that EtOH: Ammonia (9:2, v/v) is the most appropriate mobile phase for qualitatively separating of Rhodamine B, Amaranth, Red Allure, Erythrosine, and Ponceau 4R. The result showed that 51% of elementary schools at Sukolilo district, Surabaya allow the food stall presence around the school area, while the remaining not. None of the samples contain Rhodamine B. While amaranth is found in some of the comparable ketchup products. Therefore, periodical inspection by an authorized body is needed to keep the producer, i.e. food industry, strictly fit the regulations. In case of red dyes, Erythrosine is the mostly used in the ketchup, while Ponceau 4R and red allure are only used in some of the ketchup and corned beef.
KW - Amaranth
KW - Red dyes
KW - Rhodamine B
KW - Sausage
KW - TLC
UR - http://www.scopus.com/inward/record.url?scp=85059040401&partnerID=8YFLogxK
U2 - 10.1063/1.5082448
DO - 10.1063/1.5082448
M3 - Conference contribution
AN - SCOPUS:85059040401
T3 - AIP Conference Proceedings
BT - 3rd International Seminar on Chemistry
A2 - Kurniawan, Fredy
A2 - Kusumawati, Yuly
A2 - Fatmawati, Sri
A2 - Juwono, Hendro
A2 - Purnomo, Adi Setyo
PB - American Institute of Physics Inc.
T2 - 3rd International Seminar on Chemistry: Green Chemistry and Its Role for Sustainability, ISOC 2018
Y2 - 18 July 2018 through 19 July 2018
ER -