Development of Nested-PCR Assay for The Rapid Detection of Aspergillus fumigatus

Maharani Pertiwi Koentjoro; Taufik Hidayat; Suprapto Maat; Abdul Hakim Zakkiy Fasya; Endry Nugroho Prasetyo

doi:10.1063/5.0111758

Development of Nested-PCR Assay for The Rapid Detection of Aspergillus fumigatus

Maharani Pertiwi Koentjoro^*
, Taufik Hidayat
, Suprapto Maat
, Abdul Hakim Zakkiy Fasya
, Endry Nugroho Prasetyo

^*Corresponding author for this work

Laboratory of Microbiology and Biotechnology

Nahdlatul Ulama University Surabaya
Institut Teknologi Sepuluh Nopember

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

Aspergillus fumigatus is a fungal pathogen that causes Aspergillosis. This infection can develop rapidly in the human body and often causes death for immunocompromised people. Aspergillus's conidia are easily distributed in the environment and enter the paranasal sinuses or lungs. Identification of A. fumigatus is very important as an effort to prevent the spread of this fungal pathogen early. A molecular-based rapid method is promised to identify A. fumigatus without relying on morphological observations. This study represents the initial process to achieve a consensus method of nested Polymerase Chain Reaction (PCR) to diagnose A. fumigatus infection. The methods were implemented include the collection of A. fumigatus isolates, macroscopic and microscopic characterization. DNA isolation were carried out to obtain the total genome of A. fumigatus. Primers were designed to amplify 630 bp of internal transcribed spacer (ITS). To enhance the sensitivity, another primer pair was designed to amplify a 400 bp region of ITS gene and was used in a nested PCR assay. In conclusion, this initial process of nested PCR may be utilized to detect fungal A. fumigatus which can aid the diagnosis of invasive fungal disease.

Original language	English
Title of host publication	4th International Conference on Life Science and Technology, ICoLiST 2023
Editors	Ahmad Taufiq, Hendra Susanto, I. Ching Wang, Hadi Nur, Muhammad Aziz, Chuang-Rung Chang, Gereon Elbers, Sri Rahayu Lestari, Markus Diantoro, Nandang Mufti, Chen Siang Ng, Nik Ahmad Nizam Nik Malek, Sunaryono, Yu-Hsin Chiu, Siti Zubaidah, Aulanni'am, Indra Wibowo, Adeodatus Yuda Handaya
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735443136
DOIs	https://doi.org/10.1063/5.0111758
Publication status	Published - 24 Jan 2023
Event	4th International Conference on Life Science and Technology, ICoLiST 2023 - Virtual, Online, Indonesia Duration: 31 Aug 2021 → …

Publication series

Name	AIP Conference Proceedings
Volume	2634
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	4th International Conference on Life Science and Technology, ICoLiST 2023
Country/Territory	Indonesia
City	Virtual, Online
Period	31/08/21 → …

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1063/5.0111758

Cite this

Koentjoro, M. P., Hidayat, T., Maat, S., Fasya, A. H. Z., & Prasetyo, E. N. (2023). Development of Nested-PCR Assay for The Rapid Detection of Aspergillus fumigatus. In A. Taufiq, H. Susanto, I. C. Wang, H. Nur, M. Aziz, C.-R. Chang, G. Elbers, S. R. Lestari, M. Diantoro, N. Mufti, C. S. Ng, N. A. N. N. Malek, Sunaryono, Y.-H. Chiu, S. Zubaidah, Aulanni'am, I. Wibowo, & A. Y. Handaya (Eds.), 4th International Conference on Life Science and Technology, ICoLiST 2023 Article 020009 (AIP Conference Proceedings; Vol. 2634). American Institute of Physics Inc.. https://doi.org/10.1063/5.0111758

Koentjoro, Maharani Pertiwi ; Hidayat, Taufik ; Maat, Suprapto et al. / Development of Nested-PCR Assay for The Rapid Detection of Aspergillus fumigatus. 4th International Conference on Life Science and Technology, ICoLiST 2023. editor / Ahmad Taufiq ; Hendra Susanto ; I. Ching Wang ; Hadi Nur ; Muhammad Aziz ; Chuang-Rung Chang ; Gereon Elbers ; Sri Rahayu Lestari ; Markus Diantoro ; Nandang Mufti ; Chen Siang Ng ; Nik Ahmad Nizam Nik Malek ; Sunaryono ; Yu-Hsin Chiu ; Siti Zubaidah ; Aulanni'am ; Indra Wibowo ; Adeodatus Yuda Handaya. American Institute of Physics Inc., 2023. (AIP Conference Proceedings).

@inproceedings{5de0ddbea4594220b1cec4ace96782ca,

title = "Development of Nested-PCR Assay for The Rapid Detection of Aspergillus fumigatus",

abstract = "Aspergillus fumigatus is a fungal pathogen that causes Aspergillosis. This infection can develop rapidly in the human body and often causes death for immunocompromised people. Aspergillus's conidia are easily distributed in the environment and enter the paranasal sinuses or lungs. Identification of A. fumigatus is very important as an effort to prevent the spread of this fungal pathogen early. A molecular-based rapid method is promised to identify A. fumigatus without relying on morphological observations. This study represents the initial process to achieve a consensus method of nested Polymerase Chain Reaction (PCR) to diagnose A. fumigatus infection. The methods were implemented include the collection of A. fumigatus isolates, macroscopic and microscopic characterization. DNA isolation were carried out to obtain the total genome of A. fumigatus. Primers were designed to amplify 630 bp of internal transcribed spacer (ITS). To enhance the sensitivity, another primer pair was designed to amplify a 400 bp region of ITS gene and was used in a nested PCR assay. In conclusion, this initial process of nested PCR may be utilized to detect fungal A. fumigatus which can aid the diagnosis of invasive fungal disease.",

author = "Koentjoro, \{Maharani Pertiwi\} and Taufik Hidayat and Suprapto Maat and Fasya, \{Abdul Hakim Zakkiy\} and Prasetyo, \{Endry Nugroho\}",

note = "Publisher Copyright: {\textcopyright} 2023 American Institute of Physics Inc.. All rights reserved.; 4th International Conference on Life Science and Technology, ICoLiST 2023 ; Conference date: 31-08-2021",

year = "2023",

month = jan,

day = "24",

doi = "10.1063/5.0111758",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Ahmad Taufiq and Hendra Susanto and Wang, \{I. Ching\} and Hadi Nur and Muhammad Aziz and Chuang-Rung Chang and Gereon Elbers and Lestari, \{Sri Rahayu\} and Markus Diantoro and Nandang Mufti and Ng, \{Chen Siang\} and Malek, \{Nik Ahmad Nizam Nik\} and Sunaryono and Yu-Hsin Chiu and Siti Zubaidah and Aulanni'am and Indra Wibowo and Handaya, \{Adeodatus Yuda\}",

booktitle = "4th International Conference on Life Science and Technology, ICoLiST 2023",

}

Koentjoro, MP, Hidayat, T, Maat, S, Fasya, AHZ & Prasetyo, EN 2023, Development of Nested-PCR Assay for The Rapid Detection of Aspergillus fumigatus. in A Taufiq, H Susanto, IC Wang, H Nur, M Aziz, C-R Chang, G Elbers, SR Lestari, M Diantoro, N Mufti, CS Ng, NANN Malek, Sunaryono, Y-H Chiu, S Zubaidah, Aulanni'am, I Wibowo & AY Handaya (eds), 4th International Conference on Life Science and Technology, ICoLiST 2023., 020009, AIP Conference Proceedings, vol. 2634, American Institute of Physics Inc., 4th International Conference on Life Science and Technology, ICoLiST 2023, Virtual, Online, Indonesia, 31/08/21. https://doi.org/10.1063/5.0111758

Development of Nested-PCR Assay for The Rapid Detection of Aspergillus fumigatus. / Koentjoro, Maharani Pertiwi; Hidayat, Taufik; Maat, Suprapto et al.
4th International Conference on Life Science and Technology, ICoLiST 2023. ed. / Ahmad Taufiq; Hendra Susanto; I. Ching Wang; Hadi Nur; Muhammad Aziz; Chuang-Rung Chang; Gereon Elbers; Sri Rahayu Lestari; Markus Diantoro; Nandang Mufti; Chen Siang Ng; Nik Ahmad Nizam Nik Malek; Sunaryono; Yu-Hsin Chiu; Siti Zubaidah; Aulanni'am; Indra Wibowo; Adeodatus Yuda Handaya. American Institute of Physics Inc., 2023. 020009 (AIP Conference Proceedings; Vol. 2634).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Development of Nested-PCR Assay for The Rapid Detection of Aspergillus fumigatus

AU - Koentjoro, Maharani Pertiwi

AU - Hidayat, Taufik

AU - Maat, Suprapto

AU - Fasya, Abdul Hakim Zakkiy

AU - Prasetyo, Endry Nugroho

PY - 2023/1/24

Y1 - 2023/1/24

N2 - Aspergillus fumigatus is a fungal pathogen that causes Aspergillosis. This infection can develop rapidly in the human body and often causes death for immunocompromised people. Aspergillus's conidia are easily distributed in the environment and enter the paranasal sinuses or lungs. Identification of A. fumigatus is very important as an effort to prevent the spread of this fungal pathogen early. A molecular-based rapid method is promised to identify A. fumigatus without relying on morphological observations. This study represents the initial process to achieve a consensus method of nested Polymerase Chain Reaction (PCR) to diagnose A. fumigatus infection. The methods were implemented include the collection of A. fumigatus isolates, macroscopic and microscopic characterization. DNA isolation were carried out to obtain the total genome of A. fumigatus. Primers were designed to amplify 630 bp of internal transcribed spacer (ITS). To enhance the sensitivity, another primer pair was designed to amplify a 400 bp region of ITS gene and was used in a nested PCR assay. In conclusion, this initial process of nested PCR may be utilized to detect fungal A. fumigatus which can aid the diagnosis of invasive fungal disease.

AB - Aspergillus fumigatus is a fungal pathogen that causes Aspergillosis. This infection can develop rapidly in the human body and often causes death for immunocompromised people. Aspergillus's conidia are easily distributed in the environment and enter the paranasal sinuses or lungs. Identification of A. fumigatus is very important as an effort to prevent the spread of this fungal pathogen early. A molecular-based rapid method is promised to identify A. fumigatus without relying on morphological observations. This study represents the initial process to achieve a consensus method of nested Polymerase Chain Reaction (PCR) to diagnose A. fumigatus infection. The methods were implemented include the collection of A. fumigatus isolates, macroscopic and microscopic characterization. DNA isolation were carried out to obtain the total genome of A. fumigatus. Primers were designed to amplify 630 bp of internal transcribed spacer (ITS). To enhance the sensitivity, another primer pair was designed to amplify a 400 bp region of ITS gene and was used in a nested PCR assay. In conclusion, this initial process of nested PCR may be utilized to detect fungal A. fumigatus which can aid the diagnosis of invasive fungal disease.

UR - https://www.scopus.com/pages/publications/85147277481

U2 - 10.1063/5.0111758

DO - 10.1063/5.0111758

M3 - Conference contribution

AN - SCOPUS:85147277481

T3 - AIP Conference Proceedings

BT - 4th International Conference on Life Science and Technology, ICoLiST 2023

A2 - Taufiq, Ahmad

A2 - Susanto, Hendra

A2 - Wang, I. Ching

A2 - Nur, Hadi

A2 - Aziz, Muhammad

A2 - Chang, Chuang-Rung

A2 - Elbers, Gereon

A2 - Lestari, Sri Rahayu

A2 - Diantoro, Markus

A2 - Mufti, Nandang

A2 - Ng, Chen Siang

A2 - Malek, Nik Ahmad Nizam Nik

A2 - Sunaryono, null

A2 - Chiu, Yu-Hsin

A2 - Zubaidah, Siti

A2 - Aulanni'am, null

A2 - Wibowo, Indra

A2 - Handaya, Adeodatus Yuda

PB - American Institute of Physics Inc.

T2 - 4th International Conference on Life Science and Technology, ICoLiST 2023

Y2 - 31 August 2021

ER -

Koentjoro MP, Hidayat T, Maat S, Fasya AHZ, Prasetyo EN. Development of Nested-PCR Assay for The Rapid Detection of Aspergillus fumigatus. In Taufiq A, Susanto H, Wang IC, Nur H, Aziz M, Chang CR, Elbers G, Lestari SR, Diantoro M, Mufti N, Ng CS, Malek NANN, Sunaryono, Chiu YH, Zubaidah S, Aulanni'am, Wibowo I, Handaya AY, editors, 4th International Conference on Life Science and Technology, ICoLiST 2023. American Institute of Physics Inc. 2023. 020009. (AIP Conference Proceedings). doi: 10.1063/5.0111758

Development of Nested-PCR Assay for The Rapid Detection of Aspergillus fumigatus

Abstract

Publication series

Conference

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this