@inproceedings{2b3f3235ac524db0ae524d6cb3c10402,
title = "Diversity of heavy metal resistant bacteria from Kalimas Surabaya: A phylogenetic taxonomy approach",
abstract = "Bacterial resistance to heavy metal is a genetic and physiological adaptation to the environment which contaminated by heavy metal. Kalimas is an important river in Surabaya that is contaminated by some heavy metals and probably as a habitat for heavy metal resistance bacteria. Bacterial resistance to heavy metals are different for each species, and their diversity can be studied by phylogenetic taxonomy approach. Isolates screening was done using nutrient agar which contained 1 mg/L HgCl2, CdCl2 and K2Cr2O7. Bacterial viability were observed by nutrient broth which contained 10 mg/L HgCl2, 30 mg/L CdCl2 and 50 mg/L K2Cr2O7. Isolates that resistant to heavy metal and viable after exposure to heavy metal were identified using 16S rRNA gene marker by Polymerase Chain Reaction (PCR). Phylogenetic tree reconstruction was done by the neighbor-joining algorithm. Genetic assignment showed isolates that resist and viable after exposure of Hg, Cd and Cr are Bacillus S1, SS19 and DA11. Based on BLAST analysis from NCBI gene bank, 16S rRNA sequences, those isolates were similar with the member of Bacillus cereus. Depend on 16S rRNA nucleotide alignment by the neighbor-joining algorithm, Bacillus S1, SS19 and DA11 were belong to Bacillus cereus sensu-lato group.",
keywords = "16 rRNA, Becillius cereus, Phylogenetic, heavy metal",
author = "Enny Zulaika and Utomo, {Andry Prio} and Adisya Prima and Alami, {Nur Hidayatul} and Kuswytasari, {Nengah Dwianita} and Maya Shovitri and Langkah Sembiring",
note = "Publisher Copyright: {\textcopyright} 2017 Author(s).; 3rd International Biology Conference 2016: Biodiversity and Biotechnology for Human Welfare, IBOC 2016 ; Conference date: 15-10-2016",
year = "2017",
month = jun,
day = "26",
doi = "10.1063/1.4985433",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
booktitle = "Proceeding of International Biology Conference 2016",
}