Economical Wet extraction of lipid from labyrinthula aura ntiochytrium limacinum by using liquefied dimethyl ether

Rintaro Hoshino, Kazuya Murakami, Wahyudiono, Siti Machmudah, Yuji Okita, Eiji Ohashi, Hideki Kanda*, Motonobu Goto

*Corresponding author for this work

Research output: Contribution to conferencePaperpeer-review

Abstract

Recently, a simple method for the extraction of lipids from wet microalgae using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Herein, the versatility of this method was evaluated using Aurantiochytrium limacinum at 0.51 MPa and 20°C. Direct extraction of lipids from A. limacinum of moisture-rich microorganism was successfully achieved, in a lipid extraction yield of 46.1 wt% of the dry weight of the sample. On the other hands, the yields of lipid were 21.3 wt%, 43.6 wt% and 50.7 wt% by supercritical carbon dioxide (SCCO2) extraction, Hexane-Soxhlet extraction and Bligh-Dyer (BD) extraction as conventional method. However, SCCO2, hexane and BD methods, which incorporates drying and cell-disruption. The carbon and hydrogen content were improved after DME extraction. In addition, 86.7 wt% of water in the wet A. limacinum was removed. Compared with other extraction methods, liquefied DME combines drying, cell-disruption, and solvent extraction into one step.

Original languageEnglish
Pages372-380
Number of pages9
Publication statusPublished - 2015
Event13th International Symposium on East Asian Resources Recycling Technology, EARTH 2015 - Pattaya, Thailand
Duration: 1 Nov 20154 Nov 2015

Conference

Conference13th International Symposium on East Asian Resources Recycling Technology, EARTH 2015
Country/TerritoryThailand
CityPattaya
Period1/11/154/11/15

Keywords

  • Aurantiochytrium limacinum
  • Bio fuel
  • Dewatering
  • Fatty acid
  • Liquefied dimethyl ether
  • Supercritical carbon dioxide

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