Economical wet extraction of lipid from labyrinthula Aurantiochytrium limacinum by using liquefied ddimethyl ether

Rintaro Hoshino, Kazuya Murakami, Wahyudiono, Siti Machmudah, Yuji Okita, Eiji Ohashi, Hideki Kanda*, Motonobu Goto

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Recently, a simple method for the extraction of lipids from wet biomass using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Here, the versatility of this method was evaluated for labyrinthula Aurantiochytrium limacinum (A. limacinum). The liquefied DME was passed through the extractor that filled by A. limacinum at different time intervals. The extraction of lipids from A. limacinum of moisture-rich microorganism was successfully achieved, the yield of lipid was 46.1 wt% of the dry weight of the sample. In comparison, the yields of lipid were 21.3 wt%, 43.6 wt% and 50.7 wt% when supercritical carbon dioxide (SCCO2), hexane-Soxhlet and Bligh-Dyer (BD) extraction methods were applied as extractants, respectively. However, the drying and cell-disruption process were required in SCCO2, hexane-Soxhlet, and BD extraction methods.

Original languageEnglish
Pages (from-to)145-153
Number of pages9
JournalEngineering Journal
Volume20
Issue number4
DOIs
Publication statusPublished - 1 Aug 2016

Keywords

  • Aurantiochytrium limacinum
  • Bio fuel
  • Dewatering
  • Fatty acid
  • Liquefied dimethyl ether
  • Supercritical carbon dioxide

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