TY - JOUR
T1 - Economical wet extraction of lipid from labyrinthula Aurantiochytrium limacinum by using liquefied ddimethyl ether
AU - Hoshino, Rintaro
AU - Murakami, Kazuya
AU - Wahyudiono,
AU - Machmudah, Siti
AU - Okita, Yuji
AU - Ohashi, Eiji
AU - Kanda, Hideki
AU - Goto, Motonobu
N1 - Publisher Copyright:
© 2016 Chulalongkorn University 1. All rights reserved.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Recently, a simple method for the extraction of lipids from wet biomass using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Here, the versatility of this method was evaluated for labyrinthula Aurantiochytrium limacinum (A. limacinum). The liquefied DME was passed through the extractor that filled by A. limacinum at different time intervals. The extraction of lipids from A. limacinum of moisture-rich microorganism was successfully achieved, the yield of lipid was 46.1 wt% of the dry weight of the sample. In comparison, the yields of lipid were 21.3 wt%, 43.6 wt% and 50.7 wt% when supercritical carbon dioxide (SCCO2), hexane-Soxhlet and Bligh-Dyer (BD) extraction methods were applied as extractants, respectively. However, the drying and cell-disruption process were required in SCCO2, hexane-Soxhlet, and BD extraction methods.
AB - Recently, a simple method for the extraction of lipids from wet biomass using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Here, the versatility of this method was evaluated for labyrinthula Aurantiochytrium limacinum (A. limacinum). The liquefied DME was passed through the extractor that filled by A. limacinum at different time intervals. The extraction of lipids from A. limacinum of moisture-rich microorganism was successfully achieved, the yield of lipid was 46.1 wt% of the dry weight of the sample. In comparison, the yields of lipid were 21.3 wt%, 43.6 wt% and 50.7 wt% when supercritical carbon dioxide (SCCO2), hexane-Soxhlet and Bligh-Dyer (BD) extraction methods were applied as extractants, respectively. However, the drying and cell-disruption process were required in SCCO2, hexane-Soxhlet, and BD extraction methods.
KW - Aurantiochytrium limacinum
KW - Bio fuel
KW - Dewatering
KW - Fatty acid
KW - Liquefied dimethyl ether
KW - Supercritical carbon dioxide
UR - http://www.scopus.com/inward/record.url?scp=84984911004&partnerID=8YFLogxK
U2 - 10.4186/ej.2016.20.4.145
DO - 10.4186/ej.2016.20.4.145
M3 - Article
AN - SCOPUS:84984911004
SN - 0125-8281
VL - 20
SP - 145
EP - 153
JO - Engineering Journal
JF - Engineering Journal
IS - 4
ER -