Effect of gamma 60Co irradiation on the lipid content and fatty acid composition of Nannochloropsis sp. microalgae

Dini Ermavitalini*, Ika Puspita Sari, Endry Nugroho Prasetyo, Nurlita Abdulgani, Triono Bagus Saputro

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

7 Citations (Scopus)

Abstract

Nannochloropsissp. has been identified as a promising feed stock for biodiesel production in recent years. Nannochloropsis sp. have high lipid content between 31-68 % of dry biomass weight. Mutagenesis induced by Gamma 60Co irradiation can be utilized to alter Nannochloropsis sp. characteristic to get microalgae mutant strain that can produce a higher lipid content than the wild strain. The aim of this research was to know the effect of gamma 60Co irradiation to the biomass, lipid content and fatty acid compotition of Nannochloropsis sp. microalgae. In this research, irradiation was done in different doses there were 0, 2, 4, 6 and 10 Gray (Gy). Measuring microalgae biomass and lipid content were done in late exponential phase at the end of each treatment. Research design used is random complete with 95 % confident level for quantitative analysis based on biomass and lipid content. Fatty acid compotition was analyzed by gas chromatography-mass spectrometry (GC-MS). Results showed that gamma irradiated gave an effect on lipid content and fatty acid profile of Nannochloropsis sp. Tukey test showed total lipid content of control micoalgae (0 Gy) was significat different with 10 Gy irradiated microalgae, but no significant different with 2,4 and 6 Gy irradiated microalgae. The highest lipid content were found in 10 Gy irradiated microalgae equal to 62.65 %. Fatty acid profile of 10 Gy irradiated Nannochloropsis sp. had 9 fatty acids while control Nannochloropsis sp. had 6 fatty acids.

Original languageEnglish
Title of host publicationProceeding of International Biology Conference 2016
Subtitle of host publicationBiodiversity and Biotechnology for Human Welfare
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735415287
DOIs
Publication statusPublished - 26 Jun 2017
Event3rd International Biology Conference 2016: Biodiversity and Biotechnology for Human Welfare, IBOC 2016 - Surabaya, Indonesia
Duration: 15 Oct 2016 → …

Publication series

NameAIP Conference Proceedings
Volume1854
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Biology Conference 2016: Biodiversity and Biotechnology for Human Welfare, IBOC 2016
Country/TerritoryIndonesia
CitySurabaya
Period15/10/16 → …

Keywords

  • Gamma rays irradiation
  • Nannochloropsis sp.
  • biomass
  • total lipid content and fatty acid profile

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