TY - GEN
T1 - Effect of gamma 60Co irradiation on the lipid content and fatty acid composition of Nannochloropsis sp. microalgae
AU - Ermavitalini, Dini
AU - Sari, Ika Puspita
AU - Prasetyo, Endry Nugroho
AU - Abdulgani, Nurlita
AU - Saputro, Triono Bagus
N1 - Publisher Copyright:
© 2017 Author(s).
PY - 2017/6/26
Y1 - 2017/6/26
N2 - Nannochloropsissp. has been identified as a promising feed stock for biodiesel production in recent years. Nannochloropsis sp. have high lipid content between 31-68 % of dry biomass weight. Mutagenesis induced by Gamma 60Co irradiation can be utilized to alter Nannochloropsis sp. characteristic to get microalgae mutant strain that can produce a higher lipid content than the wild strain. The aim of this research was to know the effect of gamma 60Co irradiation to the biomass, lipid content and fatty acid compotition of Nannochloropsis sp. microalgae. In this research, irradiation was done in different doses there were 0, 2, 4, 6 and 10 Gray (Gy). Measuring microalgae biomass and lipid content were done in late exponential phase at the end of each treatment. Research design used is random complete with 95 % confident level for quantitative analysis based on biomass and lipid content. Fatty acid compotition was analyzed by gas chromatography-mass spectrometry (GC-MS). Results showed that gamma irradiated gave an effect on lipid content and fatty acid profile of Nannochloropsis sp. Tukey test showed total lipid content of control micoalgae (0 Gy) was significat different with 10 Gy irradiated microalgae, but no significant different with 2,4 and 6 Gy irradiated microalgae. The highest lipid content were found in 10 Gy irradiated microalgae equal to 62.65 %. Fatty acid profile of 10 Gy irradiated Nannochloropsis sp. had 9 fatty acids while control Nannochloropsis sp. had 6 fatty acids.
AB - Nannochloropsissp. has been identified as a promising feed stock for biodiesel production in recent years. Nannochloropsis sp. have high lipid content between 31-68 % of dry biomass weight. Mutagenesis induced by Gamma 60Co irradiation can be utilized to alter Nannochloropsis sp. characteristic to get microalgae mutant strain that can produce a higher lipid content than the wild strain. The aim of this research was to know the effect of gamma 60Co irradiation to the biomass, lipid content and fatty acid compotition of Nannochloropsis sp. microalgae. In this research, irradiation was done in different doses there were 0, 2, 4, 6 and 10 Gray (Gy). Measuring microalgae biomass and lipid content were done in late exponential phase at the end of each treatment. Research design used is random complete with 95 % confident level for quantitative analysis based on biomass and lipid content. Fatty acid compotition was analyzed by gas chromatography-mass spectrometry (GC-MS). Results showed that gamma irradiated gave an effect on lipid content and fatty acid profile of Nannochloropsis sp. Tukey test showed total lipid content of control micoalgae (0 Gy) was significat different with 10 Gy irradiated microalgae, but no significant different with 2,4 and 6 Gy irradiated microalgae. The highest lipid content were found in 10 Gy irradiated microalgae equal to 62.65 %. Fatty acid profile of 10 Gy irradiated Nannochloropsis sp. had 9 fatty acids while control Nannochloropsis sp. had 6 fatty acids.
KW - Gamma rays irradiation
KW - Nannochloropsis sp.
KW - biomass
KW - total lipid content and fatty acid profile
UR - http://www.scopus.com/inward/record.url?scp=85023179700&partnerID=8YFLogxK
U2 - 10.1063/1.4985400
DO - 10.1063/1.4985400
M3 - Conference contribution
AN - SCOPUS:85023179700
T3 - AIP Conference Proceedings
BT - Proceeding of International Biology Conference 2016
PB - American Institute of Physics Inc.
T2 - 3rd International Biology Conference 2016: Biodiversity and Biotechnology for Human Welfare, IBOC 2016
Y2 - 15 October 2016
ER -