Effect of laccase oxidation on phenol content and antioxidant capacity of roasted coffee

Gina Fachniar, Maharani Pertiwi Koentjoro, Isdiantoni, Ida Ekawati, Endry Nugroho Prasetyo*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The endogenous enzyme in fresh coffee beans mostly is from the class of oxidoreductase. However, those enzymes have low effectiveness in phenolic compound oxidation. The fermentation of conventional coffee processing requires a longer time. The ability of laccase could shorten the time of fermentation. Therefore, need exogenous enzyme such as laccase. Laccase is an enzyme that able to degrade lignin. In this study laccase from Trametes versicolor is effective for phenol oxidation and able to reduce bitterness in coffee beans. The bitterness of coffee beans is caused mostly by phenolic compounds in coffee such as chlorogenic acid,caffeic acid, and ferulic acid. This study aims to determine laccase oxidation in coffee beans quality by measuring phenol content and antioxidant capacity. The results showed that the lowest total phenol (0.73 ± 0.103 mg/ml) was achieved by laccase oxidation using ABTS as a mediator with antioxidant capacity level as 91.58 ± 0.001%. The benefit of this research is making the best quality of coffee beans with high antioxidant capacity and low phenol content so that it does not require added sweeteners or sugar that can interfere with health.

Original languageEnglish
Title of host publication3rd International Conference on Mathematics and Science Education, ICoMSE 2019
Subtitle of host publicationStrengthening Mathematics and Science Education Research for the Challenge of Global Society
EditorsHabiddin Habiddin, Sheikha Majid, Ibnu Suhadi, Nani Farida, I. Wayan Dasna
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419681
DOIs
Publication statusPublished - 1 Apr 2020
Event3rd International Conference on Mathematics and Science Education: Strengthening Mathematics and Science Education Research for the Challenge of Global Society, ICoMSE 2019 - Malang, East Java, Indonesia
Duration: 26 Aug 201928 Aug 2019

Publication series

NameAIP Conference Proceedings
Volume2215
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Conference on Mathematics and Science Education: Strengthening Mathematics and Science Education Research for the Challenge of Global Society, ICoMSE 2019
Country/TerritoryIndonesia
CityMalang, East Java
Period26/08/1928/08/19

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