TY - GEN
T1 - Effect of laccase oxidation pre-treatment on coffee (Coffea arabica) bean processing waste for composting substrate
AU - Nazilah, Kun Rohmatan
AU - Koentjoro, Maharani Pertiwi
AU - Isdiantoni,
AU - Ekawati, Ida
AU - Muslihatin, Wirdhatul
AU - Prasetyo, Endry Nugroho
N1 - Publisher Copyright:
© 2020 Author(s).
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Laccase oxidation pre-treatment on coffee pulp and husk in this study was expected to improve the quality of compost by removing phenol compounds which can be toxic for microorganisms. The purpose of this study was to determine the effect of pre-treatment of laccase oxidation on coffee bean processing waste (Coffea arabica) consisted pulp and husk by determining the total phenol, reducing sugar, organic materials and compost quality. Optimization of laccase production was carried out on 5 types of fermentation medium. All parameters except organic materials were measured spectrophotometrically. The results showed that the highest laccase activity (1081.2 U/ml) was achieved by E medium fermentation which contained 0.45 g of rice husk and 0.1 g glucose as carbon source, some of minerals and without the addition of sawdust and CuSO4. The lowest total phenol and reducing sugar were achieved coffee pulp and husk with laccase oxidation and ABTS pre-treatment as 0.007 ± 0.07 mg/ml and 3.29 ± 0.19 ppm respectively. It can be concluded that indeed pre-treatment of laccase oxidation did not affect the levels of organic matters. All experiment group of pre-treatment showed higher of Total Plate Counts (TPC) comparing to the control. However, the initial oxidation treatment did not have an effect on the levels of N, P, K, C, and the C / N ratio of compost produced when compared to controls. The results of this study could contribute to the process of making compost with the best quality that is environmentally friendly.
AB - Laccase oxidation pre-treatment on coffee pulp and husk in this study was expected to improve the quality of compost by removing phenol compounds which can be toxic for microorganisms. The purpose of this study was to determine the effect of pre-treatment of laccase oxidation on coffee bean processing waste (Coffea arabica) consisted pulp and husk by determining the total phenol, reducing sugar, organic materials and compost quality. Optimization of laccase production was carried out on 5 types of fermentation medium. All parameters except organic materials were measured spectrophotometrically. The results showed that the highest laccase activity (1081.2 U/ml) was achieved by E medium fermentation which contained 0.45 g of rice husk and 0.1 g glucose as carbon source, some of minerals and without the addition of sawdust and CuSO4. The lowest total phenol and reducing sugar were achieved coffee pulp and husk with laccase oxidation and ABTS pre-treatment as 0.007 ± 0.07 mg/ml and 3.29 ± 0.19 ppm respectively. It can be concluded that indeed pre-treatment of laccase oxidation did not affect the levels of organic matters. All experiment group of pre-treatment showed higher of Total Plate Counts (TPC) comparing to the control. However, the initial oxidation treatment did not have an effect on the levels of N, P, K, C, and the C / N ratio of compost produced when compared to controls. The results of this study could contribute to the process of making compost with the best quality that is environmentally friendly.
UR - http://www.scopus.com/inward/record.url?scp=85083254024&partnerID=8YFLogxK
U2 - 10.1063/5.0000880
DO - 10.1063/5.0000880
M3 - Conference contribution
AN - SCOPUS:85083254024
T3 - AIP Conference Proceedings
BT - 3rd International Conference on Mathematics and Science Education, ICoMSE 2019
A2 - Habiddin, Habiddin
A2 - Majid, Sheikha
A2 - Suhadi, Ibnu
A2 - Farida, Nani
A2 - Dasna, I. Wayan
PB - American Institute of Physics Inc.
T2 - 3rd International Conference on Mathematics and Science Education: Strengthening Mathematics and Science Education Research for the Challenge of Global Society, ICoMSE 2019
Y2 - 26 August 2019 through 28 August 2019
ER -