TY - GEN
T1 - Effect of LED Light and Different Plant Growth Regulators (PGRs) on Organogenesis of Weevil-wort (Curculigo latifolia) In vitro
AU - Sholihah, May Fiatus
AU - Rifani, Amalia Salsabila
AU - Muslihatin, Wirdhatul
AU - Jadid, Nurul
N1 - Publisher Copyright:
© 2025 American Institute of Physics Inc.. All rights reserved.
PY - 2025/1/30
Y1 - 2025/1/30
N2 - One of the obstacles in the cultivation of weevil wort (Curculigo latifolia) is its seed recalcitrance and high variability among its accessions. Plant tissue culture is an alternative technology to provide high-quality C. latifolia seeds. This study aims to determine the effect of LED lighting and plant growth regulators (PGRs) on the organogenesis of weevil-wort (C. latifolia) in vitro. Seeds of C. latifolia were planted in the Murashige & Skoog (MS0) media; MS supplemented with IBA (1 mg L-1) and BAP (2 mg L-1); and BAP alone (2 mg L-1). All culture media were then treated with different types of LED light: red, blue, and white light. Our results showed that 100% of seeds placed on the MS0 media were able to germinate in all types of LED light treatment. About 67% of seeds grown in the MS supplemented with IBA+BAP and treated with blue light were also able to develop shoots. We noticed that treatment on MS with BAP alone and LED red light showed no shoot-regenerated seed. The best performance on shoot height was obtained in the MS0 media treated with all LED light, which ranges from 1.5 - 1.87 cm. Meanwhile, the lowest shoot height of C. latifolia was obtained when the seeds were placed on MS with BAP alone (2 mg L-1).
AB - One of the obstacles in the cultivation of weevil wort (Curculigo latifolia) is its seed recalcitrance and high variability among its accessions. Plant tissue culture is an alternative technology to provide high-quality C. latifolia seeds. This study aims to determine the effect of LED lighting and plant growth regulators (PGRs) on the organogenesis of weevil-wort (C. latifolia) in vitro. Seeds of C. latifolia were planted in the Murashige & Skoog (MS0) media; MS supplemented with IBA (1 mg L-1) and BAP (2 mg L-1); and BAP alone (2 mg L-1). All culture media were then treated with different types of LED light: red, blue, and white light. Our results showed that 100% of seeds placed on the MS0 media were able to germinate in all types of LED light treatment. About 67% of seeds grown in the MS supplemented with IBA+BAP and treated with blue light were also able to develop shoots. We noticed that treatment on MS with BAP alone and LED red light showed no shoot-regenerated seed. The best performance on shoot height was obtained in the MS0 media treated with all LED light, which ranges from 1.5 - 1.87 cm. Meanwhile, the lowest shoot height of C. latifolia was obtained when the seeds were placed on MS with BAP alone (2 mg L-1).
UR - https://www.scopus.com/pages/publications/85217626065
U2 - 10.1063/5.0234911
DO - 10.1063/5.0234911
M3 - Conference contribution
AN - SCOPUS:85217626065
T3 - AIP Conference Proceedings
BT - AIP Conference Proceedings
A2 - Taufiq, Ahmad
A2 - Susanto, Hendra
A2 - Chang, Chuang-Rung
A2 - Wang, I. Ching
A2 - Nur, Hadi
A2 - Aziz, Muhammad
A2 - Lestari, Sri Rahayu
A2 - Diantoro, Markus
A2 - Mufti, Nandang
A2 - Malek, Nik Ahmad Nizam Nik
A2 - Sunaryono, null
A2 - Zubaidah, Siti
A2 - Aulanni'am, null
A2 - Wibowo, Indra
A2 - Handaya, Adeodatus Yuda
PB - American Institute of Physics
T2 - 5th International Conference on Life Science and Technology, ICoLiST 2022
Y2 - 30 August 2022 through 31 August 2022
ER -