Effect of solvent polarity levels on separation of xanthone and coumarin from Calophyllum inophyllum leaves extract

D. F. Susanto, S. Hapsari, Z. Trilutfiani, A. Borhet, H. W. Aparamarta, A. Widjaja, S. Gunawan*

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

2 Citations (Scopus)

Abstract

Calophyllum inophyllum has various benefits that can be utilized from root, stem, leaf, until seed. C. inophyllum leaves contain many bioactive compounds, such as xanthone and coumarin which are useful as antioxidant, and inhibitors of enzyme activity from HIV virus. The aim of this research was to investigate the effect of solvent polarity levels on the separation of xanthone and coumarin compounds contained in the crude extract of C. inophyllum leaves. Crude leaves extract was obtained by percolation method. Moreover, Liquid Liquid Extraction (LLE) was used for separating xanthone and coumarin compounds. It was performed by methanol (polar solvent) and hexane (non-polar solvent) with solvent ratio of 1. Methanol concentration in water used were 20%, 50%, 80%, and 100%. Each fraction obtained was tested qualitatively using Thin Layer Chromatography (TLC) and quantitatively using Gas Chromatography (GC) to analyze xanthone and coumarin. The best separation result was obtained by using 50% methanol. In this results, coumarin and xanthones were separated in methanol fraction (81.18% recovery) and in hexane fraction (81.91% recovery), respectively.

Original languageEnglish
Article number012071
JournalIOP Conference Series: Materials Science and Engineering
Volume334
Issue number1
DOIs
Publication statusPublished - 6 Apr 2018
Event3rd International Conference on Chemical Engineering Sciences and Applications 2017, ICChESA 2017 - Banda Aceh, Indonesia
Duration: 20 Sept 201721 Sept 2017

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