TY - JOUR
T1 - Effect of solvent polarity levels on separation of xanthone and coumarin from Calophyllum inophyllum leaves extract
AU - Susanto, D. F.
AU - Hapsari, S.
AU - Trilutfiani, Z.
AU - Borhet, A.
AU - Aparamarta, H. W.
AU - Widjaja, A.
AU - Gunawan, S.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2018/4/6
Y1 - 2018/4/6
N2 - Calophyllum inophyllum has various benefits that can be utilized from root, stem, leaf, until seed. C. inophyllum leaves contain many bioactive compounds, such as xanthone and coumarin which are useful as antioxidant, and inhibitors of enzyme activity from HIV virus. The aim of this research was to investigate the effect of solvent polarity levels on the separation of xanthone and coumarin compounds contained in the crude extract of C. inophyllum leaves. Crude leaves extract was obtained by percolation method. Moreover, Liquid Liquid Extraction (LLE) was used for separating xanthone and coumarin compounds. It was performed by methanol (polar solvent) and hexane (non-polar solvent) with solvent ratio of 1. Methanol concentration in water used were 20%, 50%, 80%, and 100%. Each fraction obtained was tested qualitatively using Thin Layer Chromatography (TLC) and quantitatively using Gas Chromatography (GC) to analyze xanthone and coumarin. The best separation result was obtained by using 50% methanol. In this results, coumarin and xanthones were separated in methanol fraction (81.18% recovery) and in hexane fraction (81.91% recovery), respectively.
AB - Calophyllum inophyllum has various benefits that can be utilized from root, stem, leaf, until seed. C. inophyllum leaves contain many bioactive compounds, such as xanthone and coumarin which are useful as antioxidant, and inhibitors of enzyme activity from HIV virus. The aim of this research was to investigate the effect of solvent polarity levels on the separation of xanthone and coumarin compounds contained in the crude extract of C. inophyllum leaves. Crude leaves extract was obtained by percolation method. Moreover, Liquid Liquid Extraction (LLE) was used for separating xanthone and coumarin compounds. It was performed by methanol (polar solvent) and hexane (non-polar solvent) with solvent ratio of 1. Methanol concentration in water used were 20%, 50%, 80%, and 100%. Each fraction obtained was tested qualitatively using Thin Layer Chromatography (TLC) and quantitatively using Gas Chromatography (GC) to analyze xanthone and coumarin. The best separation result was obtained by using 50% methanol. In this results, coumarin and xanthones were separated in methanol fraction (81.18% recovery) and in hexane fraction (81.91% recovery), respectively.
UR - http://www.scopus.com/inward/record.url?scp=85046270424&partnerID=8YFLogxK
U2 - 10.1088/1757-899X/334/1/012071
DO - 10.1088/1757-899X/334/1/012071
M3 - Conference article
AN - SCOPUS:85046270424
SN - 1757-8981
VL - 334
JO - IOP Conference Series: Materials Science and Engineering
JF - IOP Conference Series: Materials Science and Engineering
IS - 1
M1 - 012071
T2 - 3rd International Conference on Chemical Engineering Sciences and Applications 2017, ICChESA 2017
Y2 - 20 September 2017 through 21 September 2017
ER -
