Abstract
The objective of this research was to study the effect of various solvent extractions on the specific amino acids as small peptide or free amino acids that was contained in the extract after removal of the macromolecule protein of black soybean sprouts. The experimental design of this research was randomized complete design with one factor, which was the three various solvent, i.e. hexane, ethanol and water. The black soybean seed was germinated for 36 h. The small peptide and free amino acids of black soybean sprout were isolated at 3 various of solvents extraction, and then the macromolecule proteins in the extracts were precipitated at the pH 4. The extracts of black soybean sprout after removal of the macromolecule protein were analysed by HPLC to determine the profile of amino acids for stimulation of insulin secretion. The result of this research showed that the extracts contained the small peptide and free amino acid for stimulation of insulin secretion. The best solvent extraction was water that was due to the content of Leu, Arg, Ala, Phe, Ile, and Lys of water extract was higher than hexane and ethanol extracts.
| Original language | English |
|---|---|
| Article number | 012002 |
| Journal | IOP Conference Series: Earth and Environmental Science |
| Volume | 102 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 31 Jan 2018 |
| Externally published | Yes |
| Event | 2nd International Symposium on Food and Agro-Biodiversity, ISFA 2017 - Semarang, Indonesia Duration: 26 Sept 2017 → 27 Sept 2017 |
Keywords
- amino acids
- black soybean
- insulin
- solvents
- sprout