Effects of Organic Substance on Denitrification Efficiency

S. N. Kholida, S. Mangkoedihardjo*

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review


Toxicity can refer to the impact on all organisms, such as animals, bacteria, or plants. Other effects can occur in the substructure of an organism, such as cells or organs. This shows that toxicity can cause problems for the environment. In this study artificial sample, denitrifier and several reagents were used to analyze the main parameters, namely Dissolved Oxygen (DO), pH, Nitrate (NO3-N). The variables used were variations in nitrate concentration, process variation, and administration of selenium as a catalyst. Variations in nitrate concentration used were 20 mg/L, 40 mg/L, 40 mg/L, 60 mg/L, 80 mg/L, and 100 mg/L. The process variations used were the autotroph and heterotroph processes. The reactor used was a batch system denitrification reactor. The microorganism used was Thiobacillus denitrificans. Research data were taken every day for Dissolved Oxygen (DO), pH, Nitrate (NO3-N). This study aims to determine the efficiency of the denitrification process by autotrophs and heterotrophs using both catalysts and without catalysts. Of the two types of processes, autotrophic denitrification was efficient for processing with low nitrate concentrations. The highest efficiency value reached was 89%. Denitrification of heterotrophs was better able to process nitrates with high concentrations. The value of efficiency using this process reached 92%.

Original languageEnglish
Article number012015
JournalIOP Conference Series: Earth and Environmental Science
Issue number1
Publication statusPublished - 10 Jun 2020
EventJoint International Conference on Civil, Environmental, and Geo Engineering 2019, JIC-CEGE 2019 - Surabaya, Indonesia
Duration: 1 Oct 20192 Oct 2019


  • Denitrification
  • Efficiency
  • Organic Substance
  • Toxicity


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