TY - JOUR
T1 - Excreted cytoplasmic proteins contribute to pathogenicity in Staphylococcus aureus
AU - Ebner, Patrick
AU - Rinker, Janina
AU - Nguyen, Minh Thu
AU - Popella, Peter
AU - Nega, Mulugeta
AU - Luqman, Arif
AU - Schittek, Birgit
AU - Di Marco, Moreno
AU - Stevanovic, Stefan
AU - Götza, Friedrich
N1 - Publisher Copyright:
© 2016, American Society for Microbiology.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Excretion of cytoplasmic proteins in pro- and eukaryotes, also referred to as "nonclassical protein export," is a well-known phenomenon. However, comparatively little is known about the role of the excreted proteins in relation to pathogenicity. Here, the impact of two excreted glycolytic enzymes, aldolase (FbaA) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), on pathogenicity was investigated in Staphylococcus aureus. Both enzymes bound to certain host matrix proteins and enhanced adherence of the bacterial cells to host cells but caused a decrease in host cell invasion. FbaA and GAPDH also bound to the cell surfaces of staphylococcal cells by interaction with the major autolysin, Atl, that is involved in host cell internalization. Surprisingly, FbaA showed high cytotoxicity to both MonoMac 6 (MM6) and HaCaT cells, while GAPDH was cytotoxic only for MM6 cells. Finally, the contribution of external FbaA and GAPDH to S. aureus pathogenicity was confirmed in an insect infection model.
AB - Excretion of cytoplasmic proteins in pro- and eukaryotes, also referred to as "nonclassical protein export," is a well-known phenomenon. However, comparatively little is known about the role of the excreted proteins in relation to pathogenicity. Here, the impact of two excreted glycolytic enzymes, aldolase (FbaA) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), on pathogenicity was investigated in Staphylococcus aureus. Both enzymes bound to certain host matrix proteins and enhanced adherence of the bacterial cells to host cells but caused a decrease in host cell invasion. FbaA and GAPDH also bound to the cell surfaces of staphylococcal cells by interaction with the major autolysin, Atl, that is involved in host cell internalization. Surprisingly, FbaA showed high cytotoxicity to both MonoMac 6 (MM6) and HaCaT cells, while GAPDH was cytotoxic only for MM6 cells. Finally, the contribution of external FbaA and GAPDH to S. aureus pathogenicity was confirmed in an insect infection model.
UR - http://www.scopus.com/inward/record.url?scp=84971504912&partnerID=8YFLogxK
U2 - 10.1128/IAI.00138-16
DO - 10.1128/IAI.00138-16
M3 - Article
C2 - 27001537
AN - SCOPUS:84971504912
SN - 0019-9567
VL - 84
SP - 1672
EP - 1681
JO - Infection and Immunity
JF - Infection and Immunity
IS - 6
ER -