Exploring the potential of halotolerant bacteria for biodegradation of polycyclic aromatic hydrocarbon

Dunia A. Al Farraj; Tony Hadibarata; Adhi Yuniarto; Roua M. Alkufeidy; Maryam K. Alshammari; Achmad Syafiuddin

doi:10.1007/s00449-020-02415-4

Exploring the potential of halotolerant bacteria for biodegradation of polycyclic aromatic hydrocarbon

Dunia A. Al Farraj, Tony Hadibarata^*, Adhi Yuniarto, Roua M. Alkufeidy, Maryam K. Alshammari, Achmad Syafiuddin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The present study aimed to determine the degradation and transformation of three-ring PAHs phenanthrene and anthracene by Cryptococcus sp. MR22 and Halomonas sp. BR04 under halophilic conditions. The growth progress of Cryptococcus sp. MR22 and Halomonas sp. BR04 on anthracene and phenanthrene was monitored by colony-forming unit (CFU) technique. The growth of the bacteria was maintained at a maximum concentration of 200 mg/L of all tested hydrocarbon, indicating that Cryptococcus sp. MR22 and Halomonas sp. BR04 significantly perform in the removal of the PAH-contaminated medium at low concentrations. The fit model to represent the biodegradation kinetics of both PAHs was first-order rate equation The extract prepared from cells supplemented with three different substrates exhibited some enzymes such as hydroxylase, dioxygenase, laccase and peroxidase. The results suggest that both strains had an impressive ability in the degradation of aromatic and aliphatic hydrocarbon but also could tolerate in the extreme salinity condition.

Original language	English
Pages (from-to)	2305-2314
Number of pages	10
Journal	Bioprocess and Biosystems Engineering
Volume	43
Issue number	12
DOIs	https://doi.org/10.1007/s00449-020-02415-4
Publication status	Published - 1 Dec 2020

Keywords

Cryptococcus sp. MR22
Halomonas sp. BR04
Halotolerant bacterial
Metabolites
Phenanthrene

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title = "Exploring the potential of halotolerant bacteria for biodegradation of polycyclic aromatic hydrocarbon",

abstract = "The present study aimed to determine the degradation and transformation of three-ring PAHs phenanthrene and anthracene by Cryptococcus sp. MR22 and Halomonas sp. BR04 under halophilic conditions. The growth progress of Cryptococcus sp. MR22 and Halomonas sp. BR04 on anthracene and phenanthrene was monitored by colony-forming unit (CFU) technique. The growth of the bacteria was maintained at a maximum concentration of 200 mg/L of all tested hydrocarbon, indicating that Cryptococcus sp. MR22 and Halomonas sp. BR04 significantly perform in the removal of the PAH-contaminated medium at low concentrations. The fit model to represent the biodegradation kinetics of both PAHs was first-order rate equation The extract prepared from cells supplemented with three different substrates exhibited some enzymes such as hydroxylase, dioxygenase, laccase and peroxidase. The results suggest that both strains had an impressive ability in the degradation of aromatic and aliphatic hydrocarbon but also could tolerate in the extreme salinity condition.",

keywords = "Cryptococcus sp. MR22, Halomonas sp. BR04, Halotolerant bacterial, Metabolites, Phenanthrene",

author = "{Al Farraj}, {Dunia A.} and Tony Hadibarata and Adhi Yuniarto and Alkufeidy, {Roua M.} and Alshammari, {Maryam K.} and Achmad Syafiuddin",

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AU - Al Farraj, Dunia A.

AU - Hadibarata, Tony

AU - Yuniarto, Adhi

AU - Alkufeidy, Roua M.

AU - Alshammari, Maryam K.

AU - Syafiuddin, Achmad

PY - 2020/12/1

Y1 - 2020/12/1

N2 - The present study aimed to determine the degradation and transformation of three-ring PAHs phenanthrene and anthracene by Cryptococcus sp. MR22 and Halomonas sp. BR04 under halophilic conditions. The growth progress of Cryptococcus sp. MR22 and Halomonas sp. BR04 on anthracene and phenanthrene was monitored by colony-forming unit (CFU) technique. The growth of the bacteria was maintained at a maximum concentration of 200 mg/L of all tested hydrocarbon, indicating that Cryptococcus sp. MR22 and Halomonas sp. BR04 significantly perform in the removal of the PAH-contaminated medium at low concentrations. The fit model to represent the biodegradation kinetics of both PAHs was first-order rate equation The extract prepared from cells supplemented with three different substrates exhibited some enzymes such as hydroxylase, dioxygenase, laccase and peroxidase. The results suggest that both strains had an impressive ability in the degradation of aromatic and aliphatic hydrocarbon but also could tolerate in the extreme salinity condition.

AB - The present study aimed to determine the degradation and transformation of three-ring PAHs phenanthrene and anthracene by Cryptococcus sp. MR22 and Halomonas sp. BR04 under halophilic conditions. The growth progress of Cryptococcus sp. MR22 and Halomonas sp. BR04 on anthracene and phenanthrene was monitored by colony-forming unit (CFU) technique. The growth of the bacteria was maintained at a maximum concentration of 200 mg/L of all tested hydrocarbon, indicating that Cryptococcus sp. MR22 and Halomonas sp. BR04 significantly perform in the removal of the PAH-contaminated medium at low concentrations. The fit model to represent the biodegradation kinetics of both PAHs was first-order rate equation The extract prepared from cells supplemented with three different substrates exhibited some enzymes such as hydroxylase, dioxygenase, laccase and peroxidase. The results suggest that both strains had an impressive ability in the degradation of aromatic and aliphatic hydrocarbon but also could tolerate in the extreme salinity condition.

KW - Cryptococcus sp. MR22

KW - Halomonas sp. BR04

KW - Halotolerant bacterial

KW - Metabolites

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IS - 12

ER -

Exploring the potential of halotolerant bacteria for biodegradation of polycyclic aromatic hydrocarbon

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Keywords

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