TY - GEN
T1 - Extraction and purification of bromelain enzyme from queen pineapple
AU - Triastuti, Warlinda Eka
AU - Putra, Achmad Ferdiansyah Pradana
AU - Pudjiastuti, Lily
AU - Suprapto, Suprapto
AU - Sari, Odina Nadya
AU - Provito, Wahyu Tri Amaliah
AU - Kholifatur, A. Elyfianti
N1 - Publisher Copyright:
© 2021 Author(s).
PY - 2021/4/22
Y1 - 2021/4/22
N2 - The purpose of this study is to produce bromelain powder from extract of pineapples. Bromelain enzyme extracted by enzyme isolation method then purified by ammonium sulphate salt, ethanol and ultrafiltration membranes. Then filtering and drying is done using a vacuum dryer. The variables of ammonium sulfate salt concentrations are 10%, 15%, 20%, 25%, and 30%. The properties and bromelain activity of the pineapple extract was determined. The best result of bromelain enzyme powder from pineapple were precipitated by ammonium sulphate 15%, and ethanol 5% using membranes with activity enzyme are 0,658 U / gr and protein content were 2,03%.
AB - The purpose of this study is to produce bromelain powder from extract of pineapples. Bromelain enzyme extracted by enzyme isolation method then purified by ammonium sulphate salt, ethanol and ultrafiltration membranes. Then filtering and drying is done using a vacuum dryer. The variables of ammonium sulfate salt concentrations are 10%, 15%, 20%, 25%, and 30%. The properties and bromelain activity of the pineapple extract was determined. The best result of bromelain enzyme powder from pineapple were precipitated by ammonium sulphate 15%, and ethanol 5% using membranes with activity enzyme are 0,658 U / gr and protein content were 2,03%.
UR - http://www.scopus.com/inward/record.url?scp=85105471866&partnerID=8YFLogxK
U2 - 10.1063/5.0045354
DO - 10.1063/5.0045354
M3 - Conference contribution
AN - SCOPUS:85105471866
T3 - AIP Conference Proceedings
BT - International Conference on Chemical Science and Technology, ICCST 2020
A2 - Liu, Shih-Jung
A2 - Wirjosentono, Basuki
A2 - Sembriring, Seri Bima
A2 - Kaban, Jamaran
A2 - Marpaung, Lamek
A2 - Gea, Saharman
A2 - Basyuni, Mohammad
A2 - Mahmud, Taifo
PB - American Institute of Physics Inc.
T2 - 2020 International Conference on Chemical Science and Technology: Chemical Science and Technology Innovation for a Better Future, ICCST 2020
Y2 - 8 September 2020
ER -