Extraction and solubility evaluation of functional seed oil in supercritical carbon dioxide

Karin Tomita, Siti Machmudah, Armando T. Quitain, Mitsuru Sasaki, Ryuichi Fukuzato, Motonobu Goto*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)


Hemp (Cannabis sativa L.) seed oil is valued for its nutritional properties and for the health benefits associated with it. Its greatest feature is that the ratio of linoleic acid and linolenic acid is the desirable value of 3:1. In this research, supercritical carbon dioxide was applied to extraction of functional oil from hemp seed. In order to determine the effect of temperature and pressure on the yield of extracted components, the oil was extracted from hemp seed at temperatures between 40 and 80°C, pressures of 20-40 MPa and a CO2 flow rate of 3 mL/min. The solubility of hemp seed oil in SCCO2 determined experimentally was fitted to the Chrastil equation to determine the model parameters. The solubility calculated by Chrastil equation was compared with the experimental data. Finally, the fatty acid profile of the oil was evaluated by gas chromatography-flame ionization detection (GC-FID). There are no significant differences in the compositions of five abundant fatty acid components of the oil obtained at different sampling times with SCCO2 extraction and other extraction methods.

Original languageEnglish
Pages (from-to)109-113
Number of pages5
JournalJournal of Supercritical Fluids
Publication statusPublished - 2013
Externally publishedYes


  • Chrastil model
  • Fatty acids
  • Hemp seed
  • Solubility


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