This research was conducted to fabricate PVA/TiO2 nanofibers based on ITO glass substrates and to determine the effect of TiO2 material on the morphology of the nanofibers formed. In this study, the electrospinning method was used to fabricate nanofibers. This method utilizes a high voltage source that is connected to both poles between the needle and the collector, resulting in an electrostatic field that can attract the solution to the collector. The nanofiber characterization was carried out using an optical digital microscope. PVA/TiO2 nanofibers have been successfully fabricated on ITO glass and there is an effect of the presence of TiO2 material in the solution on the nanofiber measurement results from the side view.