The potential of Andrographis paniculata L. Ness medicinal plant extract as protector of ultraviolet radiation have been analyzed using fluorescence spectrophotometry methods. The Andrographis paniculata L. Ness medicinal plant extract was obtained by maceration process from part of the leaves. The solvent used in maceration process are ethanol as a polar solvent, ethyl acetate as a semi-polar solvent and n-hexane as a nonpolar solvent. The maceration process is carried out for 3, 5, 7 and 9 days at room temperature. The Andrographis paniculata L. Ness medicinal plant extract was characterized using FTIR spectrophotometer and analyzed by fluorescence spectrophotometry methods. The result of FTIR spectra obtained that extract has a molecular bond stretch between molecular stretch OH on 3337.38cm-1 and 3362.46cm-1. Asymmetric strain = CH2 2926.57cm-1 and the asymmetric strain of the -CH group at 2853.29cm-1. The strain of C = C ring at 1454.99 cm-1 and the O-C = O bond at 1375.91cm-1. The optimum extraction results obtained at maceration using ethanol with the excitation fluorescence intensity of 265.72 and the emission of 259.56. The optimum maceration time was obtained at 7 days using ethanol solvent as polar solvent with intensity of excitation fluorescence of 594.73 and emission of 583.34. The results of fluorescence analysis show that the Andrographis paniculata L. Ness medicinal plant extract has potential as a protector of ultraviolet radiation with optimum absorption at 282 nm which is ultraviolet area and re-emitted at 568 nm which is a visible area that is safe and harmless to the skin.