Genetic Identification of blactx-M Gene and blatem Gene on Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli from Dogs

Luviana Kristianingtyas; Mustofa Helmi Effendi; Wiwiek Tyasningsih; Fredy Kurniawan

Genetic Identification of bla_ctx-M Gene and bla_tem Gene on Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli from Dogs

Luviana Kristianingtyas, Mustofa Helmi Effendi^*, Wiwiek Tyasningsih, Fredy Kurniawan

^*Corresponding author for this work

Universitas Airlangga

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

The purpose of this study was to identify the ESBL producing E. coli and the characteristics of the encoding genes. 84 rectal dog swabs from several animal clinics in Surabaya, Indonesia were taken for the study. All the 84 positive E. coli samples were confirmed by the Double Disc Synergy Test (DDST) method. Molecular identification of bla_CTX-M and bla_TEM ESBL encoding genes using mutiplex Polymerase Chain Reaction (PCR). ESBL-producing E. coli bacteria from rectal swabs were found to be 9.52% (8/84). The PCR results showed that the bla_CTX-M gene consisted of 8 ESBL isolates and bla_TEM gene as many as 1 ESBL isolate which have the potential as a reservoir in spreading antibiotic resistance to humans and animals.

Original language	English
Pages (from-to)	17-21
Number of pages	5
Journal	Indian Veterinary Journal
Volume	97
Issue number	1
Publication status	Published - 2020
Externally published	Yes

Keywords

: Escherichia coli
Bla gene
Bla gene
Dogs
ESBL

Cite this

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title = "Genetic Identification of blactx-M Gene and blatem Gene on Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli from Dogs",

abstract = "The purpose of this study was to identify the ESBL producing E. coli and the characteristics of the encoding genes. 84 rectal dog swabs from several animal clinics in Surabaya, Indonesia were taken for the study. All the 84 positive E. coli samples were confirmed by the Double Disc Synergy Test (DDST) method. Molecular identification of blaCTX-M and blaTEM ESBL encoding genes using mutiplex Polymerase Chain Reaction (PCR). ESBL-producing E. coli bacteria from rectal swabs were found to be 9.52% (8/84). The PCR results showed that the blaCTX-M gene consisted of 8 ESBL isolates and blaTEM gene as many as 1 ESBL isolate which have the potential as a reservoir in spreading antibiotic resistance to humans and animals.",

keywords = ": Escherichia coli, Bla gene, Bla gene, Dogs, ESBL",

author = "Luviana Kristianingtyas and Effendi, {Mustofa Helmi} and Wiwiek Tyasningsih and Fredy Kurniawan",

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TY - JOUR

T1 - Genetic Identification of blactx-M Gene and blatem Gene on Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli from Dogs

AU - Kristianingtyas, Luviana

AU - Effendi, Mustofa Helmi

AU - Tyasningsih, Wiwiek

AU - Kurniawan, Fredy

PY - 2020

Y1 - 2020

N2 - The purpose of this study was to identify the ESBL producing E. coli and the characteristics of the encoding genes. 84 rectal dog swabs from several animal clinics in Surabaya, Indonesia were taken for the study. All the 84 positive E. coli samples were confirmed by the Double Disc Synergy Test (DDST) method. Molecular identification of blaCTX-M and blaTEM ESBL encoding genes using mutiplex Polymerase Chain Reaction (PCR). ESBL-producing E. coli bacteria from rectal swabs were found to be 9.52% (8/84). The PCR results showed that the blaCTX-M gene consisted of 8 ESBL isolates and blaTEM gene as many as 1 ESBL isolate which have the potential as a reservoir in spreading antibiotic resistance to humans and animals.

AB - The purpose of this study was to identify the ESBL producing E. coli and the characteristics of the encoding genes. 84 rectal dog swabs from several animal clinics in Surabaya, Indonesia were taken for the study. All the 84 positive E. coli samples were confirmed by the Double Disc Synergy Test (DDST) method. Molecular identification of blaCTX-M and blaTEM ESBL encoding genes using mutiplex Polymerase Chain Reaction (PCR). ESBL-producing E. coli bacteria from rectal swabs were found to be 9.52% (8/84). The PCR results showed that the blaCTX-M gene consisted of 8 ESBL isolates and blaTEM gene as many as 1 ESBL isolate which have the potential as a reservoir in spreading antibiotic resistance to humans and animals.

KW - : Escherichia coli

KW - Bla gene

KW - Dogs

KW - ESBL

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AN - SCOPUS:85079246921

SN - 0019-6479

VL - 97

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JO - Indian Veterinary Journal

JF - Indian Veterinary Journal

IS - 1

ER -

Genetic Identification of bla_ctx-M Gene and bla_tem Gene on Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli from Dogs

Abstract

Keywords

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