Abstract

One of the biomass lignocellulose derived from Empty Bunches of Palm Oil (EFB) has great potential as a source of biofuel because of its cellulose content reaches 40% of the total weight, the amount of abundant in Indonesia reached 31 million tons in 2019, and its utilization is still very limited. This study aims to find out the optimum condition for reducing sugar production after enzymatic hydrolysis using cellulase enzymes from Aspergillus niger and Trichoderma ressei (1:2). EFB that have been sieved measuring 100 mesh is delignified using a mixture of NaOH 2% and H2O2 5% at 70oC for 6 hours. Then, the process was continued by enzymatic hydrolysis at pH 5,5 condition and stirring speed of 125rpm. Variables used are temperatures (oC) 30, 40, and 60; substrates (gr) 1, 2, 3; and hydrolysis time (hours) 24, 36, and 48. The optimal operating condition (substrate ratio, temperature, and hydrolysis time) was studied using the Response Surface Methodology (RSM). The resulting R2 value is quite high at 95.59% and 91.62% for R2(adj). The highest concentration of reduced sugar (14.29 g/L) was achieved from the model developed at 37°C, a 3gr mass of EFB per 10 cm3water, and 24 hours hydrolysis.

Original languageEnglish
Title of host publicationProceedings of the International Conference on Advanced Technology and Multidiscipline, ICATAM 2021
Subtitle of host publication"Advanced Technology and Multidisciplinary Prospective Towards Bright Future" Faculty of Advanced Technology and Multidiscipline
EditorsPrihartini Widiyanti, Prastika Krisma Jiwanti, Gunawan Setia Prihandana, Ratih Ardiati Ningrum, Rizki Putra Prastio, Herlambang Setiadi, Intan Nurul Rizki
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735444423
DOIs
Publication statusPublished - 19 May 2023
Event1st International Conference on Advanced Technology and Multidiscipline: Advanced Technology and Multidisciplinary Prospective Towards Bright Future, ICATAM 2021 - Virtual, Online
Duration: 13 Oct 202114 Oct 2021

Publication series

NameAIP Conference Proceedings
Volume2536
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference1st International Conference on Advanced Technology and Multidiscipline: Advanced Technology and Multidisciplinary Prospective Towards Bright Future, ICATAM 2021
CityVirtual, Online
Period13/10/2114/10/21

Keywords

  • Aceton
  • Butanol
  • EFB
  • Enzymes
  • Ethanol
  • Hydrolysis

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