Identification of Dengue Virus From Human Blood Sera Type O Based on Spectrophotometry

Siti Mardiyah; Hendro Juwono; Hartono Kahar; Kartika A. Madurani; Imam Susilo; Muhammad Anas; Fredy Kurniawan

doi:10.1063/5.0313774

Identification of Dengue Virus From Human Blood Sera Type O Based on Spectrophotometry

Siti Mardiyah
, Hendro Juwono
, Hartono Kahar
, Kartika A. Madurani
, Imam Susilo
, Muhammad Anas
, Fredy Kurniawan^*

^*Corresponding author for this work

Institut Teknologi Sepuluh Nopember
Universitas Muhammadiyah Surabaya
Universitas Airlangga

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Dengue fever is a tropical infectious disease that is still a problem in the world. It is caused by the dengue virus, which causes systemic symptoms such as fever, joint pain, muscle pain, and malaise. The dengue virus identification method used in this study is based on spectrophotometry. Identification was carried out using human blood sera type O from 10 dengue hemorrhagic fever (DHF) patients. Sera was taken as much as 4 μL, then added with a saline water diluent solution of 3 mL and homogenized. The mixture was measured at a maximum excitation wavelength of 276 nm and a maximum emission of 334 nm. Excitation spectra are scanned at 200- 800 nm wavelengths. Emission spectra are scanned at wavelengths 200-900 nm. Measurement was also carried out on non-infected sera. Emission spectra of dengue and noninfectious dengue sera were treated with PCA. The results of the PCA show that dengue samples can be distinguished from non-infectious samples. The results confirm that the spectrophotometry method can identify the dengue virus infection.

Original language	English
Title of host publication	AIP Conference Proceedings
Editors	Yenny Meliana, Didi Anggoro, Andri Cahyo Kumoro, Deliana Dahnum, Muhamed Yusuf Shahul Hamid, Nurul Sahida Hassan, Mahadi Bahari
Publisher	American Institute of Physics
Edition	1
ISBN (Electronic)	9780735453173
DOIs	https://doi.org/10.1063/5.0313774
Publication status	Published - 17 Feb 2026
Event	10th International Symposium on Applied Chemistry, ISAC 2024 and the 4th International Conference on Chemical Process and Product Engineering, ICCPPE 2024 in conjunction with the 11th Conference on Emerging Energy and Process Technology, CONCEPT 2024 - Hybrid, Yogyakarta, Indonesia Duration: 23 Oct 2024 → 24 Oct 2024

Publication series

Name	AIP Conference Proceedings
Number	1
Volume	3380
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	10th International Symposium on Applied Chemistry, ISAC 2024 and the 4th International Conference on Chemical Process and Product Engineering, ICCPPE 2024 in conjunction with the 11th Conference on Emerging Energy and Process Technology, CONCEPT 2024
Country/Territory	Indonesia
City	Hybrid, Yogyakarta
Period	23/10/24 → 24/10/24

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1063/5.0313774

Cite this

Mardiyah, S., Juwono, H., Kahar, H., Madurani, K. A., Susilo, I., Anas, M., & Kurniawan, F. (2026). Identification of Dengue Virus From Human Blood Sera Type O Based on Spectrophotometry. In Y. Meliana, D. Anggoro, A. C. Kumoro, D. Dahnum, M. Y. S. Hamid, N. S. Hassan, & M. Bahari (Eds.), AIP Conference Proceedings (1 ed.). Article 020003 (AIP Conference Proceedings; Vol. 3380, No. 1). American Institute of Physics. https://doi.org/10.1063/5.0313774

Mardiyah, Siti ; Juwono, Hendro ; Kahar, Hartono et al. / Identification of Dengue Virus From Human Blood Sera Type O Based on Spectrophotometry. AIP Conference Proceedings. editor / Yenny Meliana ; Didi Anggoro ; Andri Cahyo Kumoro ; Deliana Dahnum ; Muhamed Yusuf Shahul Hamid ; Nurul Sahida Hassan ; Mahadi Bahari. 1. ed. American Institute of Physics, 2026. (AIP Conference Proceedings; 1).

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title = "Identification of Dengue Virus From Human Blood Sera Type O Based on Spectrophotometry",

abstract = "Dengue fever is a tropical infectious disease that is still a problem in the world. It is caused by the dengue virus, which causes systemic symptoms such as fever, joint pain, muscle pain, and malaise. The dengue virus identification method used in this study is based on spectrophotometry. Identification was carried out using human blood sera type O from 10 dengue hemorrhagic fever (DHF) patients. Sera was taken as much as 4 μL, then added with a saline water diluent solution of 3 mL and homogenized. The mixture was measured at a maximum excitation wavelength of 276 nm and a maximum emission of 334 nm. Excitation spectra are scanned at 200- 800 nm wavelengths. Emission spectra are scanned at wavelengths 200-900 nm. Measurement was also carried out on non-infected sera. Emission spectra of dengue and noninfectious dengue sera were treated with PCA. The results of the PCA show that dengue samples can be distinguished from non-infectious samples. The results confirm that the spectrophotometry method can identify the dengue virus infection.",

author = "Siti Mardiyah and Hendro Juwono and Hartono Kahar and Madurani, \{Kartika A.\} and Imam Susilo and Muhammad Anas and Fredy Kurniawan",

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Mardiyah, S, Juwono, H, Kahar, H, Madurani, KA, Susilo, I, Anas, M & Kurniawan, F 2026, Identification of Dengue Virus From Human Blood Sera Type O Based on Spectrophotometry. in Y Meliana, D Anggoro, AC Kumoro, D Dahnum, MYS Hamid, NS Hassan & M Bahari (eds), AIP Conference Proceedings. 1 edn, 020003, AIP Conference Proceedings, no. 1, vol. 3380, American Institute of Physics, 10th International Symposium on Applied Chemistry, ISAC 2024 and the 4th International Conference on Chemical Process and Product Engineering, ICCPPE 2024 in conjunction with the 11th Conference on Emerging Energy and Process Technology, CONCEPT 2024, Hybrid, Yogyakarta, Indonesia, 23/10/24. https://doi.org/10.1063/5.0313774

Identification of Dengue Virus From Human Blood Sera Type O Based on Spectrophotometry. / Mardiyah, Siti; Juwono, Hendro; Kahar, Hartono et al.
AIP Conference Proceedings. ed. / Yenny Meliana; Didi Anggoro; Andri Cahyo Kumoro; Deliana Dahnum; Muhamed Yusuf Shahul Hamid; Nurul Sahida Hassan; Mahadi Bahari. 1. ed. American Institute of Physics, 2026. 020003 (AIP Conference Proceedings; Vol. 3380, No. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Identification of Dengue Virus From Human Blood Sera Type O Based on Spectrophotometry

AU - Mardiyah, Siti

AU - Juwono, Hendro

AU - Kahar, Hartono

AU - Madurani, Kartika A.

AU - Susilo, Imam

AU - Anas, Muhammad

AU - Kurniawan, Fredy

PY - 2026/2/17

Y1 - 2026/2/17

N2 - Dengue fever is a tropical infectious disease that is still a problem in the world. It is caused by the dengue virus, which causes systemic symptoms such as fever, joint pain, muscle pain, and malaise. The dengue virus identification method used in this study is based on spectrophotometry. Identification was carried out using human blood sera type O from 10 dengue hemorrhagic fever (DHF) patients. Sera was taken as much as 4 μL, then added with a saline water diluent solution of 3 mL and homogenized. The mixture was measured at a maximum excitation wavelength of 276 nm and a maximum emission of 334 nm. Excitation spectra are scanned at 200- 800 nm wavelengths. Emission spectra are scanned at wavelengths 200-900 nm. Measurement was also carried out on non-infected sera. Emission spectra of dengue and noninfectious dengue sera were treated with PCA. The results of the PCA show that dengue samples can be distinguished from non-infectious samples. The results confirm that the spectrophotometry method can identify the dengue virus infection.

AB - Dengue fever is a tropical infectious disease that is still a problem in the world. It is caused by the dengue virus, which causes systemic symptoms such as fever, joint pain, muscle pain, and malaise. The dengue virus identification method used in this study is based on spectrophotometry. Identification was carried out using human blood sera type O from 10 dengue hemorrhagic fever (DHF) patients. Sera was taken as much as 4 μL, then added with a saline water diluent solution of 3 mL and homogenized. The mixture was measured at a maximum excitation wavelength of 276 nm and a maximum emission of 334 nm. Excitation spectra are scanned at 200- 800 nm wavelengths. Emission spectra are scanned at wavelengths 200-900 nm. Measurement was also carried out on non-infected sera. Emission spectra of dengue and noninfectious dengue sera were treated with PCA. The results of the PCA show that dengue samples can be distinguished from non-infectious samples. The results confirm that the spectrophotometry method can identify the dengue virus infection.

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BT - AIP Conference Proceedings

A2 - Meliana, Yenny

A2 - Anggoro, Didi

A2 - Kumoro, Andri Cahyo

A2 - Dahnum, Deliana

A2 - Hamid, Muhamed Yusuf Shahul

A2 - Hassan, Nurul Sahida

A2 - Bahari, Mahadi

PB - American Institute of Physics

T2 - 10th International Symposium on Applied Chemistry, ISAC 2024 and the 4th International Conference on Chemical Process and Product Engineering, ICCPPE 2024 in conjunction with the 11th Conference on Emerging Energy and Process Technology, CONCEPT 2024

Y2 - 23 October 2024 through 24 October 2024

ER -

Mardiyah S, Juwono H, Kahar H, Madurani KA, Susilo I, Anas M et al. Identification of Dengue Virus From Human Blood Sera Type O Based on Spectrophotometry. In Meliana Y, Anggoro D, Kumoro AC, Dahnum D, Hamid MYS, Hassan NS, Bahari M, editors, AIP Conference Proceedings. 1 ed. American Institute of Physics. 2026. 020003. (AIP Conference Proceedings; 1). doi: 10.1063/5.0313774

Identification of Dengue Virus From Human Blood Sera Type O Based on Spectrophotometry

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