In vitro propagation of Indonesian stevia (Stevia rebaudiana) genotype using axenic nodal segments

Nurul Jadid*, Suci Anggraeni, Muhammad Rifqi Nur Ramadani, Mutiara Arieny, Faisol Mas’ud

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Objective: The high industrial demand for Stevia cultivation (Stevia rebaudiana) has increased due to its high stevioside content derived from the leaves. However, the low germination rate makes the cultivation of the plant become the main obstacle. Therefore, an efficient cultivation technique is required. This present work aims to analyze the effect of five combinations of Kinetin (Kin) and benzyladenine (BA) on stevia micropropagation using nodal segment explants. Results: The micropropagation of stevia was performed using Murashige and Skoog (MS) medium supplemented with BA and Kin. We analyzed different organogenesis and callogenesis responses. In addition, the number of shoots and root formed during in vitro culture were also observed. Our results demonstrated that all treatments with Kin, both alone and in combination with BA, resulted in the development of callus on all nodal segment explants. Explants treated in MS with 1 mg L−1 BA exhibited the best average of shoot number (36.27). In contrast, the treatment without PGR resulted in the best root formation (2.6). The overall results suggested that different combination of BA and Kin resulted in distinct organogenesis responses, where 1 mg L−1 of BA was potentially used for boosting the number of shoots in micropropagation of stevia accession Mini.

Original languageEnglish
Article number45
JournalBMC Research Notes
Issue number1
Publication statusPublished - Dec 2024


  • Callogenesis
  • Micropropagation
  • Organogenesis
  • Plant growth regulators
  • Stevia rebaudiana


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