Nowadays, the application of nano-sized anatase titanium dioxide particles becomes one of the essential research focuses by many experts, especially for biomedical applications. In this work, we report the utilization of coprecipitation route in preparing nano-sized anatase titanium dioxide as an antifungal agent. The data analysis for the XRD data of the titanium dioxide particles showed that the sample crystallized with anatase structure and sized in the nanometric size of 13.30 nm. The particle size obtained from Scherrer’s analysis of the XRD data was quite similar to the particle size obtained from electron microscopy investigation. Furthermore, the small-angle scattering data presented that the anatase titanium dioxide constructed a 3-dimensional structure with a compact structure originating from the fractal dimension value of approximately 3. The functional groups of the nano-sized anatase titanium dioxide were traced in the wavelength range of 500-1500 cm-1 showing the presence of Ti-O bonding. Interestingly, the prepared sample in this experiment exhibited an excellent performance as an antifungal agent represented by inhibition zone diameter of 3.59 mm.