Investigation on Fe2+-dependent Reaction on Methyl Orange Biodecolorization by Daedalea dickinsii

Adi S. Purnomo*, Kurnia Dewi, Hamdan D. Rizqi, Herdayanto S. Putro

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The investigation of Fe2+-dependent reaction on methyl orange (MO) biodegradation by brown-rot fungus Daedalea dickinsii was performed to evaluate the involvement of Fenton reaction. The MO degradation (final concentration 75 mg/L) was evaluated for incubation period at 0, 7, 14, 21, and 28 days in mineral salt media with and without Fe2+. UV-Vis Spectrophotometer and LC-TOF/MS were used for degradation analysis. The highest MO decolorization by D. dickinsii was approximately 77.1% and 60.5% in medium without and with Fe2+, respectively, occurred for 28 days incubation. It indicated that the optimum decolorization was obtained in medium without Fe2+, which assumed that Fe2+ dependent reaction might be not enhanced decolorization of MO by D. dickinsii. Based on LC-TOF/MS analysis, 2-hydroxy-4((2-hydroxy-4(methylamino) phenyl) diazinyl) phenolate (C13H12N3O3), and 4-(2-(4-(dimethylamino)-2,3,5,6-tetramethoxy phenyl) hydrazinyl)-2,3,5,6tetramethoxy benzenesulphonate (C22H32N3O11S) were detected as metabolites of MO degradation. C13H12N3O3 was a transformation product of demethylation, hydroxylation and desulfonation, while C22H32N3O11S was a product of methoxylation. This study indicated that the methyl orange (MO) decolorization was a hybrid process between Fenton's mechanism and enzymatic activity.

Original languageEnglish
Title of host publication1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021
EditorsArika Indah Kristiana, Ridho Alfarisi
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735443006
DOIs
Publication statusPublished - 4 Jan 2023
Event1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021 - Jember, Indonesia
Duration: 18 Sept 202119 Sept 2021

Publication series

NameAIP Conference Proceedings
Volume2679
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021
Country/TerritoryIndonesia
CityJember
Period18/09/2119/09/21

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