Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum

The highest population of Calophyllum inophyllum is in Indonesia. One of the bioactive compound contained in C. inophyllum leave is cholestane. The extraction method was employed to obtain crude extract from these leaves. It was followed by identification of the bioactive compounds. The purpose of this research was to develop a process for isolating and identifying cholestane and dihydropyrene from methanolic extract of C. inophyllum leaves in high yield and purity. The effect of crude extract to non-polar solvent mass ratio and non-polar solvent types on the separation of the bioactive compounds were also systematically investigated. New compounds (trans-2-[2-(trifluoromethyl)phenyl]-10b,10c-dimethyl-10b, 10c-dihydropyrene and anti-4-aza-B-homo-5.alpha-cholestane-3-one) were also identified in C. inophyllum leaves. The successful separation was obtained by employing CS₂ as the solvent and crude extract to CS₂ mass ratio of 1/10 (g/g).